S100A9 anticorps (Internal Region)

N° du produit ABIN335161

Cet anticorps Chèvre Polyclonal détecte spécifiquement S100A9 dans WB, IHC, ELISA, IF et FACS. Il présente une réactivité envers Humain.

Aperçu rapide pour S100A9 anticorps (Internal Region) (ABIN335161)

Antigène: S100A9 (S100 Calcium Binding Protein A9 (S100A9))

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp S100A9 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Classe de qualité: Verified

Détail du produit anti-S100A9 anticorps

Épitope: Internal Region

Fonction: S100A9

Séquence: DTNADKQLSF EEF

Réactivité croisée: Humain

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogène: C-DTNADKQLSFEEF

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry: Paraffin embedded Human Lung. Recommended concentration: 2.5 μg/mL.

Western Blot: Approx 13-14 kDa band observed in Human Bone Marrow lysates and approx 14-15 kDa in Human Gastrointestinal cancer lysates (calculated MW of 13.2 kDa according to NP_002956.1). Recommended concentration: 0.5-2 μg/mL. Primary incubation 1 hour a

Peptide ELISA: antibody detection limit dilution 1:8000.

Commentaires:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm and nuclei of MCF7 and U2OS cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of MCF7 cells. Recommended conc

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Détails sur S100A9

Antigène: S100A9 (S100 Calcium Binding Protein A9 (S100A9))

Autre désignation: S100A9

Sujet: S100A9, S100 calcium binding protein A9, 60B8AG, CAGB, CFAG, CGLB, L1AG, LIAG, MAC387, MIF, MRP14, NIF, P14, S100 calcium binding protein A9 (calgranulin B), S100 calcium-binding protein A9, S100 calcium-binding protein A9 (calgranulin B), calgranulin B

ID gène: 6280

NCBI Accession: NP_002956

Pathways: Transition Metal Ion Homeostasis, Positive Regulation of Endopeptidase Activity, S100 Proteins