Dynactin 1 anticorps (AA 1266-1278)

N° du produit ABIN337005

Cet anticorps anti-Dynactin 1 Polyclonal Chèvre (ABIN337005) détecte spécifiquement Dynactin 1 dans WB, ELISA, IHC et IHC (p). L’anticorps est réactif avec des échantillons de Humain, Souris, Cheval, Singe et Hamster.

Aperçu rapide pour Dynactin 1 anticorps (AA 1266-1278) (ABIN337005)

Antigène: Dynactin 1 (DCTN1)

Reactivité: Humain, Souris, Cheval, Singe, Hamster

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp Dynactin 1 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-Dynactin 1 anticorps

Épitope: AA 1266-1278

Specificité: Human DCTN1. This antibody is expected to recognise all reported isoforms (NP_004073.2, NP_075408.1, NP_001128512.1, NP_001128513.1).

Homologie: Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Hamster, Horse (100%) Rat, Bovine, Dog, Panda, Rabbit, Pig (92%) Bat (85%).

Purification: Immunoaffinity purified

Immunogène: Synthetic peptide C-QEQLHQLHSRLIS from the C-terminus of human DCTN1 (NP_004073.2, NP_075408.1, NP_001128512.1, NP_001128513.1). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Hamster, Horse (100%), Rat, Bovine, Dog, Panda, Rabbit, Pig (92%), Bat (85%).

Type of Immunogen: Synthetic peptide

Information d'application

Indications d'application: Approved: IHC, IHC-P (2.5 μg/mL), Peptide-ELISA (1:128000), WB (1:128000, 0.5 - 2 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store at -20°C. Avoid freeze-thaw cycles.

Détails sur Dynactin 1

Antigène: Dynactin 1 (DCTN1)

Autre désignation: DCTN1 / Dynactin 1

Sujet: Name/Gene ID: DCTN1

Synonyms: DCTN1, DAP-150, Dynactin subunit 1, DP-150, Dynactin 1, HMN7B, p150-glued, p135

ID gène: 1639

NCBI Accession: NP_004073, NP_075408, NP_001128512, NP_001128513

UniProt: Q14203

Pathways: M Phase, ER-Nucleus Signaling