GCLC anticorps (AA 295-313)

N° du produit ABIN337056

Cet anticorps anti-GCLC Polyclonal Lapin (ABIN337056) détecte spécifiquement GCLC dans WB, IHC, ELISA, IP et IHC (p). L’anticorps est réactif avec des échantillons de Humain, Souris, Rat, Boeuf (Vache) et Singe.

Aperçu rapide pour GCLC anticorps (AA 295-313) (ABIN337056)

Antigène: GCLC (Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC))

Reactivité: Humain, Souris, Rat, Boeuf (Vache), Singe

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp GCLC est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-GCLC anticorps

Épitope: AA 295-313

Specificité: Recognizes the heavy subunit of rat GCS. Species cross-reactivity: human, monkey and mouse. Cross-reacts weakly with bovine.

Purification: Protein A purified

Immunogène: A synthetic peptide derived from corresponding to aa295-313 of rat GCLC (heavy subunit of GCS).

Type of Immunogen: Synthetic peptide

Information d'application

Indications d'application: Approved: ELISA, IHC, IHC-P (5 μg/mL), IP, WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Suitable for use in Western Blot and Immunoprecipitation. Western Blot: 2.5-5 μg/mL for 2hrs at RT. Immunoprecipitation: 10 μg/mg protein lysate. Denatured verified. Use Protein A. Positive control: Jurkat, MAD109, PC12 cells.

Commentaires:

Target Species of Antibody: Rat

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.2 % BSA, 0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C,-20 °C

Stockage commentaire: 4°C or -20°C, Avoid freeze-thaw cycles.

Détails sur GCLC

Antigène: GCLC (Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC))

Autre désignation: GCLC

Sujet: Name/Gene ID: GCLC

Synonyms: GCLC, GCL, GCS heavy chain, Gamma-ECS, GCS, GLCL, GLCLC

ID gène: 2729

UniProt: P48506

Pathways: Cell RedoxHomeostasis