Cullin 1 anticorps (C-Term)

N° du produit ABIN337174

Cet anticorps Souris Monoclonal détecte spécifiquement Cullin 1 dans WB, IHC, ELISA, IP et IHC (p). Il présente une réactivité envers Humain.

Aperçu rapide pour Cullin 1 anticorps (C-Term) (ABIN337174)

Antigène: Cullin 1 (CUL1)

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Cullin 1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-Cullin 1 anticorps

Épitope: C-Term

Marque: IHC-plus™

Specificité: Recognizes Cullin 1. Reacts specifically with the ~86kD CUL-1 protein and does not cross-react with the related CUL-2 protein. This antibody is confirmed to be reactive with human. Reactivity is confirmed from extracts of IMR90 (human fibroblasts) and Wi-38 cells.

Purification: Protein A purified

Immunogène: A synthetic peptide corresponding to an 11 amino acid sequence at the C-terminus of the human CUL-1 protein.

Type of Immunogen: Synthetic peptide

Isotype: IgG1 kappa

Information d'application

Indications d'application: Approved: ELISA (0.1 - 1 μg/mL), IHC, IHC-P (10 μg/mL), IP, WB (1 - 3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. Immunoprecipitation: 5-10 μg/ IP reaction.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeat freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Détails sur Cullin 1

Antigène: Cullin 1 (CUL1)

Autre désignation: Cullin 1 / CUL1

Sujet: Name/Gene ID: CUL1

Synonyms: CUL1, Cullin 1, Cullin-1, CUL-1

ID gène: 8454

UniProt: Q13616

Pathways: Cycle Cellulaire, Signalisation Hedgehog, Mitotic G1-G1/S Phases, Regulation of Cell Size