HSPG2 anticorps

N° du produit ABIN337194

Cet anticorps anti-HSPG2 est un anticorps Souris Monoclonal détectant HSPG2 dans WB, IHC, ELISA, IP, IHC (p) et IHC (fro). Adapté pour Humain et Boeuf (Vache).

Aperçu rapide pour HSPG2 anticorps (ABIN337194)

Antigène: HSPG2 (Heparan Sulfate Proteoglycan 2 (HSPG2))

Reactivité: Humain, Boeuf (Vache)

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp HSPG2 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: A71

Détail du produit anti-HSPG2 anticorps

Marque: IHC-plus™

Specificité: Highly specific for bovine perlecan. The epitope is located in domain I. No cross-reactivity with other connective tissue proteins (vitronectin, fibronectin, elastin, collagen, laminin). Species cross-reactivity: Human.

Purification: Protein A/G purified

Immunogène: Bovine corneal endothelial cells.

Type of Immunogen: Cells

Isotype: IgG1 kappa

Information d'application

Indications d'application: Approved: ELISA (1:4000), IHC, IHC-Fr, IHC-P (5 μg/mL), IP, WB (1:50)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Commentaires:

Target Species of Antibody: Bovine

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 15  mM sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeat freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Détails sur HSPG2

Antigène: HSPG2 (Heparan Sulfate Proteoglycan 2 (HSPG2))

Autre désignation: HSPG2 / Perlecan

Sujet: Name/Gene ID: HSPG2

Synonyms: HSPG2, Endorepellin (domain V region), HSPG, Heparan sulfate proteoglycan 2, Perlecan, Perlecan proteoglycan, SJA, SJS1, PRCAN, SJS

ID gène: 3339

UniProt: P98160

Pathways: Glycosaminoglycan Metabolic Process, Lipid Metabolism