CAMK2B anticorps (pThr286)
Aperçu rapide pour CAMK2B anticorps (pThr286) (ABIN337356)
Antigène
Voir toutes CAMK2B AnticorpsReactivité
Hôte
Clonalité
Conjugué
Application
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Épitope
- pThr286
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Specificité
- Specific for the ~50k a-CaM Kinase II and the ~60k R-CaM Kinase II proteins phosphorylated at Thr286. Immunolabeling is blocked by the X-phosphatase treatment
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Purification
- Immunoaffinity purified
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Immunogène
- Phosphopeptide corresponding to amino acid residues surrounding the phosphoThr286 found in rat brain CaM Kinase II.
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Indications d'application
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Approved: IHC, IHC-P (1:200), WB (1:1000)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 1:200. -
Commentaires
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Target Species of Antibody: Rat
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- Lot specific
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Buffer
- 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg per ml BSA, 50 % glycerol
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Conseil sur la manipulation
- Avoid repeated freezing and thawing.
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Stock
- 4 °C,-20 °C
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Stockage commentaire
- 4°C or -20°C, Avoid freeze-thaw cycles.
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- CAMK2B (Calcium/calmodulin-Dependent Protein Kinase II beta (CAMK2B))
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Autre désignation
- CAMK2 / CAMKII
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Sujet
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Name/Gene ID: CAMK2
Synonyms: CAMK2 -
Pathways
- Signalisation WNT, Interferon-gamma Pathway, Myometrial Relaxation and Contraction, Regulation of G-Protein Coupled Receptor Protein Signaling, Smooth Muscle Cell Migration, Regulation of long-term Neuronal Synaptic Plasticity
Antigène
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