Transglutaminase 2 anticorps

N° du produit ABIN337398

Cet anticorps anti-Transglutaminase 2 est un anticorps Lapin Polyclonal détectant Transglutaminase 2 dans WB, IHC, IP et IHC (p). Adapté pour Humain, Souris et Rat.

Aperçu rapide pour Transglutaminase 2 anticorps (ABIN337398)

Antigène: Transglutaminase 2 (TGM2) (Transglutaminase 2 (C Polypeptide, Protein-Glutamine-gamma-Glutamyltransferase) (TGM2))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Transglutaminase 2 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-Transglutaminase 2 anticorps

Specificité: Recognizes Transglutaminase II. Species cross-reactivity: Human, mouse, rat.

Purification: Protein A purified

Immunogène: Recombinant full length human TGase II (77-85kD). Cellular Localization: Cytoplasm, cell membrane, and extracellular matrix.

Type of Immunogen: Recombinant protein

Isotype: IgG

Information d'application

Indications d'application: Approved: IHC, IHC-P (5 μg/mL), IP (10 μg/tst), WB (5 - 10 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Immunoprecipitation (Native verified): 10 μg/mg protein lysate. Use Protein A. Western Blot: 5-10 μg/mL for 2hrs at RT. Positive control: HUVEC cells. Breast carcinoma. The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4.

Agent conservateur: Azide free

Conseil sur la manipulation: avoid freeze thaw cycles. Store undiluted.

Stock: 4 °C,-20 °C

Stockage commentaire: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Store undiluted.

Détails sur Transglutaminase 2

Antigène: Transglutaminase 2 (TGM2) (Transglutaminase 2 (C Polypeptide, Protein-Glutamine-gamma-Glutamyltransferase) (TGM2))

Autre désignation: TGM2 / Transglutaminase 2

Sujet: Name/Gene ID: TGM2

Synonyms: TGM2, G-ALPHA-h, GNAH, TG(C), Transglutaminase 2, Transglutaminase C, TG2, TGase H, TGase-2, TGase-H, Transglutaminase H, TGase C, TGC, TTG, C polypeptide, Tgase II, Tissue transglutaminase, Transglutaminase-2

ID gène: 7052

UniProt: P21980

Pathways: Tube Formation, Thromboxane A2 Receptor Signaling