CD14 anticorps

N° du produit ABIN343179

Cet anticorps anti-CD14 est un anticorps Souris Monoclonal détectant CD14 dans ELISA. Adapté pour Souris.

Aperçu rapide pour CD14 anticorps (ABIN343179)

Antigène: CD14

Reactivité: Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD14 est non-conjugé

Application: ELISA

Clone: BiG57

Détail du produit anti-CD14 anticorps

Specificité: Recombinant mouse CD14. The antibody don't blocks LPS binding to membrane bound CD14.

Purification: Protein G affinity column

Immunogène: recombinant mouse CD14

Isotype: IgG1

Information d'application

Indications d'application: ELISA , immunostaining of mouse macrophages after stimulation 6h with LPS, Western blot with recombinant mouse CD14. Binding titre at mouse CD14 transfected CHO-cells: more than 100.000 (ELISA, Flow cytometry), no binding at LPS-binding site, endotoxin content less than 0.1ng/ml

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Reconstitute with bidistilled water

Concentration: 1 mg/mL

Buffer: PBS, pH 7.15

Agent conservateur: Without preservative

Stock: -20 °C,-80 °C

Détails sur CD14

Antigène: CD14

Autre désignation: CD14

Sujet: Background: biG 57 represents an excellent marker for CD14. The CD14 glycoprotein, gp 55, is present on most monocytic and macrophages like cell types: monocytes, macrophages, weekly at surface of neutrophiles like Kupffer cells, pleural phagocytic cells and dendritic reticular cells. CD14 is also observed on granulocytes and activated or transformed B-cells. Furthermore CD14 is present in a soluble form in human serum, urine and other body fluids. The CD14 Molecule has been reported to be a receptor for endotoxin. CD14 is anchored to cells by linkage to glycosylphosphatidylinositol (GPI) and functions as a high affinity receptor for LPS-LBP (lipopolysaccharide binding protein)-complexes.

Poids moléculaire: ~50kDa

ID gène: 12475

NCBI Accession: NP_033971

UniProt: P10810

Pathways: Signalisation TLR, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades