CD14 anticorps

N° du produit ABIN343180

Cet anticorps Souris Monoclonal détecte spécifiquement CD14 dans ELISA. Il présente une réactivité envers Souris.

Aperçu rapide pour CD14 anticorps (ABIN343180)

Antigène: CD14

Reactivité: Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD14 est non-conjugé

Application: ELISA

Clone: BiG53

Détail du produit anti-CD14 anticorps

Specificité: Membrane bound and soluble mouse CD14. The antibody blocks LPS binding to membrane bound CD14.

Purification: Protein A affinity column

Immunogène: Monocytes of mouse

Isotype: IgG1

Information d'application

Indications d'application: Suitable for immunostaining of CD14 positive cells and inhibition studies Binds at LPS activated mouse monocytes as well as mouse CD14 transfected cells or recombinant mouse CD14 ELISA. Inhibition of LPS binding to CD14 (Binding at LPS binding site). Up to 1 ug/ml biG53 inhibit binding of FITC-LPS (0.5ug/ml) to 6 x 105 CD14+CHO transfectants (FACS), Binding titre at mouse CD14 transfected CHO-cells: more than 20.000 (ELISA, Flow cytometry)

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Reconstitute with bidistilled water

Concentration: 1 mg/mL

Buffer: PBS, pH 7.16

Agent conservateur: Without preservative

Stock: -20 °C,-80 °C

Détails sur CD14

Antigène: CD14

Autre désignation: CD14

Sujet: Background: biG 53 represents an excellent marker for CD14. The CD14 glycoprotein, gp 55, is present on most monocytic and macrophages like cell types: monocytes, macrophages, weekly at surface of neutrophiles like Kupffer cells, pleural phagocytic cells and dendritic reticular cells. CD14 is also observed on granulocytes and activated or transformed B-cells. Furthermore CD14 is present in a soluble form in human serum, urine and other body fluids. The CD14 Molecule has been reported to be a receptor for endotoxin. CD14 is anchored to cells by linkage to glycosylphosphatidylinositol (GPI) and functions as a high affinity receptor for LPS-LBP (lipopolysaccharide binding protein)-complexes.

ID gène: 12475

NCBI Accession: NP_033971

UniProt: P10810

Pathways: Signalisation TLR, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades