NEDD8 anticorps (N-Term)

N° du produit ABIN7698970

Cet anticorps anti-NEDD8 est un anticorps Mouton Polyclonal détectant NEDD8 dans WB, IHC et IHC (p). Adapté pour Souris.

Aperçu rapide pour NEDD8 anticorps (N-Term) (ABIN7698970)

Antigène: NEDD8 (Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8))

Reactivité: Souris

Hôte: Mouton

Clonalité: Polyclonal

Conjugué: Cet anticorp NEDD8 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-NEDD8 anticorps

Épitope: N-Term

Fonction: Sheep antibody to NEDD8

Specificité: Specific for NEDD8.

Réactivité croisée: Humain, Souris, Rat

Réactivité croisée (Details): Other species not yet tested.

Purification: IgG

Immunogène: A synthetic peptide from the n-terminal of mouse NEDD8 (Ubiquitin-like protein Nedd8 Neddylin) conjugated to immunogenic carrier protein has been used as the immunogen. The antigen is homologous in human rat zebrafish drosophila and xenopus.

Isotype: IgG

Information d'application

Indications d'application: IHC (both on frozen,paraffin embedded tissues) WB. Use at a concentration of 10-50,micro,g,ml. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Reconstitute in 500 µl of sterile water. Centrifuge to remove any insoluble material.

Conseil sur la manipulation: Avoid freeze and thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Date de péremption: 12 months

Détails sur NEDD8

Antigène: NEDD8 (Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8))

Autre désignation: NEDD8

Sujet: Function: Ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis. Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C-APPBP1 and linkage to the E2 enzyme UBE2M. Attachment of NEDD8 to cullins activates their associated E3 ubiquitin ligase activity and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory protein. Subcellular location: Mainly nuclear Tissue specificity: Ubiquitously expressed (at protein level). Post-translational modification: Cleavage of precursor form by UCHL3 or SENP8 is necessary for function

UniProt: P29595

Pathways: Ubiquitin Proteasome Pathway