Axin anticorps (AA 850-862)

N° du produit ABIN351463

Cet anticorps Chèvre Polyclonal détecte spécifiquement Axin dans ELISA, IHC et IHC (p). Il présente une réactivité avec des échantillons de Humain, Souris, Rat, Singe, Boeuf (Vache), Roussette (Chauve-souris), Hamster, Cheval et Poisson zèbre (Danio rerio).

Aperçu rapide pour Axin anticorps (AA 850-862) (ABIN351463)

Antigène: Axin (AXIN1) (Axin 1 (AXIN1))

Reactivité: Humain, Souris, Rat, Singe, Boeuf (Vache), Roussette (Chauve-souris), Hamster, Cheval, Poisson zèbre (Danio rerio)

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp Axin est non-conjugé

Application: ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-Axin anticorps

Épitope: AA 850-862

Specificité: Human AXIN1. This antibody is expected to recognise both reported isoforms.

Homologie: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Baboon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Bat, Horse, Opossum, Stickleback, Pufferfish, Zebrafish (100%) Turkey, Chicken (92%) Xenopus, Sea anemone (85%).

Purification: Immunoaffinity purified

Immunogène: Synthetic peptide C-FEEKIIGKVEKVD from the C-terminus of human AXIN1 (NP_003493.1, NP_851393.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Baboon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Bat, Horse, Opossum, Stickleback, Pufferfish, Zebrafish (100%), Turkey, Chicken (92%), Xenopus, Sea anemone (85%).

Type of Immunogen: Synthetic peptide

Information d'application

Indications d'application: Approved: IHC, IHC-P (3.75 μg/mL), Peptide-ELISA (1:128000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 3.75 μg/mL.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store at -20°C. Avoid freeze-thaw cycles.

Détails sur Axin

Antigène: Axin (AXIN1) (Axin 1 (AXIN1))

Autre désignation: AXIN1 / Axin-1

Sujet: Name/Gene ID: AXIN1

Synonyms: AXIN1, Axis inhibitor 1, AXIN, Axin 1, Axis inhibition protein 1, Fused, mouse, homolog of, HAxin, PPP1R49, Axin-1

ID gène: 8312

NCBI Accession: NP_003493, NP_851393

UniProt: O15169

Pathways: Signalisation WNT, Sensory Perception of Sound, Regulation of G-Protein Coupled Receptor Protein Signaling