GLI2 anticorps (AA 46-60)

N° du produit ABIN351530

L’anticorps anti-GLI2 Polyclonal Lapin est utilisé pour la détection de GLI2 dans des échantillons de Humain. Il a été validé pour WB, ELISA, IHC et IHC (p).

Aperçu rapide pour GLI2 anticorps (AA 46-60) (ABIN351530)

Antigène: GLI2 (GLI Family Zinc Finger 2 (GLI2))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp GLI2 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-GLI2 anticorps

Épitope: AA 46-60

Specificité: Amino acids 46-60 of human Gli-2 (isoform a).

Purification: Immunoaffinity purified

Immunogène: Corresponds to amino acids 46-60 of human Gli-2 (isoform a).

Type of Immunogen: Synthetic peptide

Information d'application

Indications d'application: Approved: ELISA (1:2000 - 1:12000), IHC, IHC-P (5 μg/mL), WB (1:500 - 1:2000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Multiple splice variants have been reported for this protein. Detection of Gli-2 by western blot may be enhanced if nuclear extracts are used instead of whole cell lysates as the expression/abundance of Gli-2 is likely to be low. Furthermore, Gli-2 expression is likely to be developmentally regulated and induced, making it difficult to detect in whole tissue homogenates.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 0.01 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeat freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Détails sur GLI2

Antigène: GLI2 (GLI Family Zinc Finger 2 (GLI2))

Autre désignation: GLI2

Sujet: Name/Gene ID: GLI2

Synonyms: GLI2, GLI-Kruppel family member GLI2, HPE9, Gli-2, Oncogene GLI2, Tax helper protein 2, THP, Zinc finger protein GLI2, Tax helper protein, THP2, GLI family zinc finger 2, Tax helper protein 1, THP1

ID gène: 2736

UniProt: P10070

Pathways: Signalisation Hedgehog, Dopaminergic Neurogenesis