PCNA anticorps

N° du produit ABIN356318

L’anticorps Souris Monoclonal anti-PCNA a été validé pour WB, IF, IHC (p) et IHC (fro). Il convient pour détecter PCNA dans des échantillons de Humain, Souris, Rat, Saccharomyces cerevisiae et Insectes. Il y a 1 publication disponible.

Aperçu rapide pour PCNA anticorps (ABIN356318)

Antigène: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Reactivité: Humain, Souris, Rat, Saccharomyces cerevisiae, Insectes

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp PCNA est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: PC10

Détail du produit anti-PCNA anticorps

Specificité: This antibody is specific for PCNA. PC10 reacts specifically with a 36 kDa nuclear protein of the Proliferating Cell Nuclear Antigen (PCNA). PCNA is an excellent marker of proliferative cells in routinely processed tissue sections.
Cellular Localization: Nuclear.

Réactivité croisée (Details): Species reactivity (tested):Human, Mouse, Rat, Insect and Yeast

Attributs du produit: Synonyms: Cyclin, Proliferating Cell Nuclear Antigen

Purification: Purified

Immunogène: Recombinant rat PCNA protein. Remarks: Molecular weight of antigen: ~30 kDa.

Isotype: IgG2a

Information d'application

Indications d'application: Suitable for Immunohistochemistry and Immunocytochemistry (Frozen or Formalin-FixedParaffin-Embedded (FFPE) tissue sections and cell smears)For IHC dilute concentared antibody at 1/50-1/200, use streptavidinapprox. biotin system orpolymer system, incubate 30 minutes at room temperature. FFPE tissue section requires antigen retriever (boiling tissue in 10 mM citrate, pH 6.0 for 20 mins, followed by cooling at RT for 20 mins). Recommended Positive Control: Human tonsil, lymph node. Western Blot: 1/100-1/400.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.2 mg/mL

Buffer: PBS, pH 7.4 containing 1 % BSA as stabilizer and 0.05 % Sodium Azide as preservative.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: One year from despatch.

Date de péremption: 12 months

Références pour anticorps anti-PCNA (ABIN356318)

Oka, Ohno, Tsuchimoto, Sakumi, Furuichi, Nakabeppu: "Two distinct pathways of cell death triggered by oxidative damage to nuclear and mitochondrial DNAs." dans: The EMBO journal, Vol. 27, Issue 2, pp. 421-32, (2008) (PubMed).

Détails sur PCNA

Antigène: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Autre désignation: PCNA

Sujet: Proliferating Cell Nuclear Antigen, commonly known as PCNA, is a protein that acts as a processivity factor for DNA polymerase delta in eukaryotic cells. The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. PCNA was originally identified as an antigen that is expressed in the nuclei of cells during the DNA synthesis phase of the cell cycle It is increased during late G1 phase and S phase of the cell cycle and declines during G2 and M phases.Synonyms: Cyclin, Proliferating Cell Nuclear Antigen

UniProt: P12004

Pathways: Telomere Maintenance, Réparation de l'ADN, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Autophagy