SMURF2 anticorps (N-Term)

N° du produit ABIN357471

Cet anticorps anti-SMURF2 Polyclonal Lapin (ABIN357471) détecte spécifiquement SMURF2 dans WB, IHC (p) et EIA. L’anticorps est réactif avec des échantillons de Humain et Souris.

Aperçu rapide pour SMURF2 anticorps (N-Term) (ABIN357471)

Antigène: SMURF2 (SMAD Specific E3 Ubiquitin Protein Ligase 2 (SMURF2))

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp SMURF2 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Détail du produit anti-SMURF2 anticorps

Épitope: N-Term

Specificité: This antibody detects SMURF2 at N-term.

Purification: Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS

Immunogène: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human SMURF2.

Isotype: Ig Fraction

Information d'application

Indications d'application: ELISA 1: 1,000. Western blot 1: 100 - 1: 500. Immunohistochemistry 1: 50 - 1: 100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.

Détails sur SMURF2

Antigène: SMURF2 (SMAD Specific E3 Ubiquitin Protein Ligase 2 (SMURF2))

Autre désignation: SMURF2

Sujet: SMURF2, a 748-amino acid ubiquitin E3 ligase that is 83 % identical to SMURF1, codes for a C2-WW-HECT domain ubiquitin ligase that associates constitutively with SMAD7. Binding to SMAD7 induces export of SMURF2 and recruitment to the activated transforming growth factor-beta receptor (TGFBR), where it causes receptor and SMAD7 degradation. A strong interaction of second and third SMURF2 WW domains has been identified with SMAD1, SMAD2, and SMAD3, but not SMAD4. Western blot analysis showed that SMURF2 selectively downregulates the transcription of SMAD2 and SMAD1, but not SMAD3. The nuclear SMURF2/phosphorylated SMAD2 interaction is requires TGFB1.Synonyms: E3 ubiquitin-protein ligase SMURF2, SMAD ubiquitination regulatory factor 2, SMAD-specific E3 ubiquitin-protein ligase 2, SMURF 2, SMURF-2

Poids moléculaire: 86195 Da

ID gène: 64750

NCBI Accession: NP_073576

UniProt: Q9HAU4