MIB1 anticorps (N-Term)

N° du produit ABIN357602

L’anticorps Lapin Polyclonal anti-MIB1 a été validé pour WB, IF, IHC (p) et EIA. Il convient pour détecter MIB1 dans des échantillons de Humain.

Aperçu rapide pour MIB1 anticorps (N-Term) (ABIN357602)

Antigène: MIB1 (Mindbomb E3 Ubiquitin Protein Ligase 1 (MIB1))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp MIB1 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Détail du produit anti-MIB1 anticorps

Épitope: N-Term

Specificité: This antibody is specific to MIB1 (N-term). Predicted to cross react with Mouse and Zebrafish (100 % Antigen Homology).

Purification: Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Immunogène: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human MIB.

Isotype: Ig Fraction

Information d'application

Indications d'application: ELISA: 1/1,000. Western Blot: 1/100-1/500. Immunohistochemistry: 1/50-1/100. Immunofluorescence: 1/20-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) Sodium Azide as preservative.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Détails sur MIB1

Antigène: MIB1 (Mindbomb E3 Ubiquitin Protein Ligase 1 (MIB1))

Autre désignation: MIB1

Sujet: MIB is an E3 ubiquitin-protein ligase that mediates ubiquitination of Delta receptors, which act as ligands of Notch proteins. This protein positively regulates the Delta-mediated Notch signaling by ubiquitinating the intracellular domain of Delta, leading to endocytosis of Delta receptors. MIB probably mediates ubiquitination and subsequent proteasomal degradation of DAPK1, thereby antagonizing anti-apoptotic effects of DAPK1 to promote TNF-induced apoptosis.Synonyms: DAPK-interacting protein 1, DIP1, E3 ubiquitin-protein ligase MIB1, KIAA1323, Mind bomb homolog 1, ZZANK2, Zinc finger ZZ type with ankyrin repeat domain protein 2

Poids moléculaire: 110135 Da

ID gène: 57534, 9606

UniProt: Q86YT6

Pathways: SARS-CoV-2 Protein Interactome, Phosphorylation & l'infection par le SRAS-CoV-2