EIF4E anticorps (pSer209)

N° du produit ABIN358406

L’anticorps anti-EIF4E Polyclonal Lapin est utilisé pour la détection de EIF4E dans des échantillons de Humain. Il a été validé pour EIA.

Aperçu rapide pour EIF4E anticorps (pSer209) (ABIN358406)

Antigène: EIF4E (Eukaryotic Translation Initiation Factor 4E (EIF4E))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp EIF4E est non-conjugé

Application: Enzyme Immunoassay (EIA)

Détail du produit anti-EIF4E anticorps

Épitope: pSer209

Specificité: This antibody detectsEIF4E pSer209.

Purification: Affinity purification in a 2-step procedure with the control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Immunogène: This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S209 of human EIF4E.

Isotype: Ig Fraction

Information d'application

Indications d'application: ELISA: 1/1,000. Dot Blot: 1/500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) Sodium Azide as preservative.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Détails sur EIF4E

Antigène: EIF4E (Eukaryotic Translation Initiation Factor 4E (EIF4E))

Autre désignation: EIF4E

Sujet: EIF4F is a multi-subunit complex, the composition of which varies with external and internal environmental conditions. It is composed of at least EIF4A, EIF4E and EIF4G1/EIF4G3. EIF4E is also known to interact with other partners. The interaction with EIF4ENIF1 mediates the import into the nucleus. Nonphosphorylated EIF4EBP1, EIF4EBP2 and EIF4EBP3 compete with EIF4G1/EIF4G3 to interact with EIF4E, insulin stimulated MAP-kinase (MAPK1 and MAPK3) phosphorylation of EIF4EBP1 causes dissociation of the complex allowing EIF4G1/EIF4G3 to bind and consequent initiation of translation. Rapamycin can attenuate insulin stimulation, mediated by FKBPs.Synonyms: EIF-4E, EIF4EL1, EIF4F, Eukaryotic translation initiation factor 4E, eIF-4F 25 kDa subunit, mRNA cap-binding protein

Poids moléculaire: 25097 Da

ID gène: 1977, 9606

UniProt: P06730

Pathways: BCR Signaling