DNMT1 anticorps (pSer714)

N° du produit ABIN358447

Cet anticorps Lapin Polyclonal détecte spécifiquement DNMT1 dans EIA. Il présente une réactivité avec des échantillons de Humain.Il a été cité dans 1 publication.

Aperçu rapide pour DNMT1 anticorps (pSer714) (ABIN358447)

Antigène: DNMT1 (DNA (Cytosine-5)-Methyltransferase 1 (DNMT1))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp DNMT1 est non-conjugé

Application: Enzyme Immunoassay (EIA)

Détail du produit anti-DNMT1 anticorps

Épitope: pSer714

Specificité: This antibody detects Dnmt1 pSer714.

Purification: Protein A Chromatography followed by two-step phosphospecific peptide affinity purification.

Immunogène: This Dnmt1 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Ser714 of human Dnmt1.

Isotype: Ig Fraction

Information d'application

Indications d'application: ELISA: 1/1,000. Dot Blot: 1/500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) Sodium Azide as preservative.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Références pour anticorps anti-DNMT1 (ABIN358447)

Dion, Lin, Hubert, Waterland, Wilson: "Dnmt1 deficiency promotes CAG repeat expansion in the mouse germline." dans: Human molecular genetics, Vol. 17, Issue 9, pp. 1306-17, (2008) (PubMed).

Détails sur DNMT1

Antigène: DNMT1 (DNA (Cytosine-5)-Methyltransferase 1 (DNMT1))

Autre désignation: DNMT1

Sujet: Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, 3 families of mammalian DNA methyltransferase genes have been identified which include Dnmt1, Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. Dnmt2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. The Dnmt3 family members, Dnmt3a and Dnmt3b, are strongly expressed in ES cells but their expression is down regulated in differentiating ES cells and is low in adult somatic tissue. Dnmt1 co-purifies with the retinoblastoma (Rb) tumour suppressor gene product, E2F1, and HDAC1. Dnmt1 also cooperates with Rb to repress transcription from promoters containing E2Fbinding sites suggesting a link between DNA methylation, histone deacetylase and sequence-specific DNA binding activity, as well as a growth-regulatory pathway that is disrupted in nearly all cancer cells.Synonyms: AIM, CXXC-type zinc finger protein 9, CXXC9, DNA (cytosine-5)-methyltransferase 1, DNA methyltransferase HsaI, HsaI, MCMT

Poids moléculaire: 183164 Da

ID gène: 1786, 9606

UniProt: P26358

Pathways: SARS-CoV-2 Protein Interactome, Phosphorylation & l'infection par le SRAS-CoV-2