NEK1 anticorps (C-Term)

N° du produit ABIN360536

L’anticorps Lapin Polyclonal anti-NEK1 a été validé pour WB, IHC (p) et EIA. Il convient pour détecter NEK1 dans des échantillons de Humain et Hamster.

Aperçu rapide pour NEK1 anticorps (C-Term) (ABIN360536)

Antigène: NEK1 (NIMA-Related Kinase 1 (NEK1))

Reactivité: Humain, Hamster

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp NEK1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Détail du produit anti-NEK1 anticorps

Épitope: AA 1173-1202, C-Term

Specificité: This antibody is specific to NEK1.

Purification: Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS

Immunogène: KLH conjugated synthetic peptide between 1173~1202 amino acids from the C-terminal region of Human NEK1.

Isotype: Ig Fraction

Information d'application

Indications d'application: ELISA: 1/1,000. Western blotting: 1/100-1/500. Immunohistochemistry: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) Sodium Azide as preservative

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Détails sur NEK1

Antigène: NEK1 (NIMA-Related Kinase 1 (NEK1))

Autre désignation: NEK1

Sujet: Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The STE group (homologs of yeast Sterile 7, 11, 20 kinases) consists of 50 kinases related to the mitogen-activated protein kinase (MAPK) cascade families (Ste7/MAP2K, Ste11/MAP3K, and Ste20/MAP4K). MAP kinase cascades, consisting of a MAPK and one or more upstream regulatory kinases (MAPKKs) have been best characterized in the yeast pheromone response pathway. Pheromones bind to Ste cell surface receptors and activate yeast MAPK pathway.Synonyms: NimA-related protein kinase 1, NimA-related protein kinase 10, Renal carcinoma antigen NY-REN-55, Serine/threonine-protein kinase Nek1

ID gène: 4750

NCBI Accession: NP_001186326

UniProt: Q96PY6