FAK anticorps (pTyr861)

N° du produit ABIN361988

Cet anticorps Lapin Polyclonal détecte spécifiquement FAK dans WB. Il présente une réactivité avec des échantillons de Humain, Souris et Rat. Il a été cité dans 4+ publications.

Aperçu rapide pour FAK anticorps (pTyr861) (ABIN361988)

Antigène: FAK (PTK2) (PTK2 Protein tyrosine Kinase 2 (PTK2))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp FAK est non-conjugé

Application: Western Blotting (WB)

Détail du produit anti-FAK anticorps

Épitope: pTyr861

Specificité: The antibody detects endogenous level of FAK only when phosphorylated at tyrosine 861.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

Immunogène: Peptide sequence around phosphorylation site of pTyr861 (H-I-Y (p) -Q-P) derived from Human FAK. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.

Isotype: IgG

Information d'application

Indications d'application: Western blotting: 1:500-1:1000

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C/-20 °C

Stockage commentaire: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

Références pour anticorps anti-FAK (ABIN361988)

Kanehira, Kikuchi, Ohkouchi, Shibahara, Tode, Santoso, Daito, Ohta, Tamada, Nukiwa: "Targeting lysophosphatidic acid signaling retards culture-associated senescence of human marrow stromal cells." dans: PLoS ONE, Vol. 7, Issue 2, pp. e32185, (2012) (PubMed).

Sheng, Song, Zheng, Zhou, Lu, Zhao, Zhang, Gong: "Abnormal cleavage of APP impairs its functions in cell adhesion and migration." dans: Neuroscience letters, Vol. 450, Issue 3, pp. 327-31, (2009) (PubMed).

Zheng, Wei, Wang, Gong, Zhang: "Surface characterization and cytocompatibility of three chitosan/polycation composite membranes for guided bone regeneration." dans: Journal of biomaterials applications, Vol. 24, Issue 3, pp. 209-29, (2009) (PubMed).

Wu, Zhu, Shi, Zhang, Ma, Yu: "Zoledronate inhibits the proliferation, adhesion and migration of vascular smooth muscle cells." dans: European journal of pharmacology, Vol. 602, Issue 1, pp. 124-31, (2008) (PubMed).

Détails sur FAK

Antigène: FAK (PTK2) (PTK2 Protein tyrosine Kinase 2 (PTK2))

Autre désignation: FAK

Sujet: Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Plays a potential role in oncogenic transformations resulting in increased kinase activity.

Poids moléculaire: 125 kDa

UniProt: Q05397

Pathways: Response to Growth Hormone Stimulus, CXCR4-mediated Signaling Events, Smooth Muscle Cell Migration, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling