CDC25A anticorps (pSer76)

N° du produit ABIN362128

Cité dans 1 publication. Cet anticorps anti-CDC25A Polyclonal Lapin (ABIN362128) détecte spécifiquement CDC25A dans WB et IHC. L’anticorps est réactif avec des échantillons de Humain, Souris et Rat.

Aperçu rapide pour CDC25A anticorps (pSer76) (ABIN362128)

Antigène: CDC25A (Cell Division Cycle 25 Homolog A (S. Pombe) (CDC25A))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CDC25A est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Détail du produit anti-CDC25A anticorps

Épitope: pSer76

Specificité: The antibody detects endogenous level of cdc25A only when phosphorylated at serine 76.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Immunogène: Peptide sequence around phosphorylation site of pSer76 (M-G-S (p) -S-E) derived from Human cdc25A. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.

Isotype: IgG

Information d'application

Indications d'application: Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C/-20 °C

Stockage commentaire: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

Références pour anticorps anti-CDC25A (ABIN362128)

Piao, Lee, Xu, Gwak, Oh, Park, Ha: "CK1ε targets Cdc25A for ubiquitin-mediated proteolysis under normal conditions and in response to checkpoint activation." dans: Cell cycle (Georgetown, Tex.), Vol. 10, Issue 3, pp. 531-7, (2011) (PubMed).

Détails sur CDC25A

Antigène: CDC25A (Cell Division Cycle 25 Homolog A (S. Pombe) (CDC25A))

Autre désignation: cdc25A

Sujet: Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Directly dephosphorylates CDC2 and stimulates its kinase activity. Also dephosphorylates CDK2 in complex with cyclin E, in vitro.

Poids moléculaire: 70 kDa

NCBI Accession: NP_001780

UniProt: P30304

Pathways: Cycle Cellulaire, Mitotic G1-G1/S Phases, M Phase