PRKAA1 anticorps (pSer487)

N° du produit ABIN362186

Cet anticorps Lapin Polyclonal détecte spécifiquement PRKAA1 dans WB, IHC et IF. Il présente une réactivité envers Humain, Souris et Rat et a été mentionné dans 1 publication.

Aperçu rapide pour PRKAA1 anticorps (pSer487) (ABIN362186)

Antigène: PRKAA1 (Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PRKAA1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Détail du produit anti-PRKAA1 anticorps

Épitope: pSer487

Specificité: The antibody detects endogenous level of AMPKa1 only when phosphorylated at serine 487.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Immunogène: Peptide sequence around phosphorylation site of pSer487 (S-G- SP-V-S) derived from Human AMPKa1. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.

Isotype: IgG

Information d'application

Indications d'application: Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100
Immunofluorescence: 1:100-1:200

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C/-20 °C

Stockage commentaire: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

Références pour anticorps anti-PRKAA1 (ABIN362186)

Huang, Qi, Xiang, Wang: "Construction of adiponectin-encoding plasmid DNA and overexpression in mice in vivo." dans: Gene, Vol. 502, Issue 2, pp. 87-93, (2012) (PubMed).

Détails sur PRKAA1

Antigène: PRKAA1 (Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1))

Autre désignation: AMPKalpha1

Sujet: Responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. Appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-AMP rises in response to fuel limitation and/or hypoxia. This is a catalytic subunit.

Poids moléculaire: 63 kDa

NCBI Accession: NP_006242

UniProt: P54646

Pathways: AMPK Signaling, Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process, L'effet Warburg