SAPK, JNK (pThr183) anticorps
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- Antigène
- SAPK, JNK
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Épitope
- pThr183
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Reactivité
- Humain, Souris, Rat
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Hôte
- Lapin
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Clonalité
- Polyclonal
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Conjugué
- Inconjugué
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Application
- Immunohistochemistry (IHC), Western Blotting (WB)
- Specificité
- The antibody detects endogenous level of SAPK/JNK only when phosphorylated at threonine 183.
- Purification
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
- Immunogène
- Peptide sequence around phosphorylation site of pThr183 (M-M-T (p) -P-Y) derived from Human SAPK, JNK. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.
- Isotype
- IgG
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- Indications d'application
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Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100 - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- 4 °C/-20 °C
- Stockage commentaire
- Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
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MK-2206 induces cell cycle arrest and apoptosis in HepG2 cells and sensitizes TRAIL-mediated cell death." dans: Molecular and cellular biochemistry, Vol. 382, Issue 1-2, pp. 217-24, (2013) (PubMed).
: "Activation by phosphorylation and purification of human c-Jun N-terminal kinase (JNK) isoforms in milligram amounts." dans: Protein expression and purification, Vol. 75, Issue 2, pp. 138-46, (2010) (PubMed).
: "
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MK-2206 induces cell cycle arrest and apoptosis in HepG2 cells and sensitizes TRAIL-mediated cell death." dans: Molecular and cellular biochemistry, Vol. 382, Issue 1-2, pp. 217-24, (2013) (PubMed).
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- Antigène
- SAPK, JNK
- Autre désignation
- SAPK/JNK
- Synonymes
- anticorps JNK-55, anticorps JNK2, anticorps JNK2A, anticorps JNK2ALPHA, anticorps JNK2B, anticorps JNK2BETA, anticorps PRKM9, anticorps SAPK, anticorps SAPK1a, anticorps p54a, anticorps p54aSAPK, anticorps AI851083, anticorps Prkm9, anticorps mitogen-activated protein kinase 9, anticorps MAPK9, anticorps Mapk9
- Sujet
- Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as c-Jun and ATF2 and thus regulates AP-1 transcriptional activity. In T-cells, JNK1 and JNK2 are required for polarized differentiation of T-helper cells into Th1 cells.
- Poids moléculaire
- 54 kDa
- NCBI Accession
- NP_001128
- UniProt
- P53779
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