Cortactin anticorps (pTyr466)

N° du produit ABIN362346

L’anticorps anti-Cortactin Polyclonal Lapin est utilisé pour la détection de Cortactin dans des échantillons de Humain et Souris. Il a été validé pour WB.

Aperçu rapide pour Cortactin anticorps (pTyr466) (ABIN362346)

Antigène: Cortactin (CTTN)

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Cortactin est non-conjugé

Application: Western Blotting (WB)

Détail du produit anti-Cortactin anticorps

Épitope: pTyr466

Specificité: The antibody detects endogenous level of Cortactin only when phosphorylated at tyrosine 466.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

Immunogène: Peptide sequence around phosphorylation site of pTyr466 (P-V-Y (p) -E-T) derived from Human CORTACTIN. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.

Isotype: IgG

Information d'application

Indications d'application: Western blotting: 1:500-1:1000

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C/-20 °C

Stockage commentaire: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

Détails sur Cortactin

Antigène: Cortactin (CTTN)

Autre désignation: Cortactin

Sujet: Cortactin is overexpressed in breast cancer and squamous cell carcinomas of the head and neck. The encoded protein is localized in the cytoplasm and in areas of the cell-substratum contacts. This gene has two roles: (1) regulating the interactions between components of adherens-type junctions and (2) organizing the cytoskeleton and cell adhesion structures of epithelia and carcinoma cells. During apoptosis, the encoded protein is degraded in a caspase-dependent manner. The aberrant regulation of this gene contributes to tumor cell invasion and metastasis. Two splice variants that encode different isoforms have been identified for this gene.

Poids moléculaire: 85 kDa

NCBI Accession: NP_005222

UniProt: Q14247

Pathways: Signalisation MAPK