RPS6KB1 anticorps (pSer424)

N° du produit ABIN362364

Il existe 1 publication pour ce produit. L’anticorps anti-RPS6KB1 Polyclonal Lapin est utilisé pour la détection de RPS6KB1 dans des échantillons de Humain, Souris et Rat. Il a été validé pour WB, IHC et IF.

Aperçu rapide pour RPS6KB1 anticorps (pSer424) (ABIN362364)

Antigène: RPS6KB1 (Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1 (RPS6KB1))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp RPS6KB1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Détail du produit anti-RPS6KB1 anticorps

Épitope: pSer424

Specificité: The antibody detects endogenous level of p70 S6 Kinase only when phosphorylated at serine 424.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

Immunogène: Peptide sequence around phosphorylation site of pSer424 (P-V-S (p) -P-V) derived from Human p70 S6 Kinase. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.

Isotype: IgG

Information d'application

Indications d'application: Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100
Immunofluorescence: 1:100-1:200

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C/-20 °C

Stockage commentaire: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

Références pour anticorps anti-RPS6KB1 (ABIN362364)

Liu, Huang, Chen, Zeng, Guo, Zhang, Liu, Xu, Mo, Li: "Activation of mammalian target of rapamycin pathway confers adverse outcome in nonsmall cell lung carcinoma." dans: Cancer, Vol. 117, Issue 16, pp. 3763-73, (2011) (PubMed).

Détails sur RPS6KB1

Antigène: RPS6KB1 (Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1 (RPS6KB1))

Autre désignation: p70 S6 Kinase

Sujet: Phosphorylates specifically ribosomal protein S6 in response to insulin or several classes of mitogens. Promotes protein synthesis by phosphorylating PDCD4 at 'Ser-67' and targeting it for degradation.

Poids moléculaire: 85 kDa

NCBI Accession: NP_003152

UniProt: P23443

Pathways: Signalisation PI3K-Akt, Signalisation RTK, AMPK Signaling, Regulation of Cell Size, Skeletal Muscle Fiber Development, Feeding Behaviour, G-protein mediated Events, Smooth Muscle Cell Migration, Interaction of EGFR with phospholipase C-gamma, L'effet Warburg