H2AFX anticorps (AA 137-141)

N° du produit ABIN362952

Détail du produit anti-H2AFX anticorps

Antigène: H2AFX (H2A Histone Family, Member X (H2AFX))

Épitope: AA 137-141

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp H2AFX est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Specificité: The antibody detects endogenous level of total Histone H2A.X protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific immunogen.

Immunogène: Peptide sequence around AA 137-141 (Q-A-S-Q-E) derived from Human Histone H2A.X. Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates.

Isotype: IgG

Information d'application

Indications d'application: Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100
Immunofluorescence: 1:100-1:200

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C/-20 °C

Stockage commentaire: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

Références pour anticorps anti-H2AFX (ABIN362952)

Qing, Liang, Du, Fan, Xu, Xu, Shi: "Apoptosis induced by trimethyltin chloride in human neuroblastoma cells SY5Y is regulated by a balance and cross-talk between NF-κB and MAPKs signaling pathways." dans: Archives of toxicology, Vol. 87, Issue 7, pp. 1273-85, (2013) (PubMed).

Li, Hu, He, Li, Zhu, Hou, Zhang, Li, Zhang, Wang, Liu, Yao, Zhang: "Up-regulation of NDRG2 through nuclear factor-kappa B is required for Leydig cell apoptosis in both human and murine infertile testes." dans: Biochimica et biophysica acta, Vol. 1822, Issue 2, pp. 301-13, (2012) (PubMed).

Li, Kikuchi, Zheng, Noguchi, Takahashi, Nishida, Masuda, Yang, Takano: "Downregulation and translocation of nuclear ING4 is correlated with tumorigenesis and progression of head and neck squamous cell carcinoma." dans: Oral oncology, Vol. 47, Issue 3, pp. 217-23, (2011) (PubMed).

Li, Nishida, Noguchi, Zheng, Takahashi, Yang, Masuda, Takano: "Decreased nuclear expression and increased cytoplasmic expression of ING5 may be linked to tumorigenesis and progression in human head and neck squamous cell carcinoma." dans: Journal of cancer research and clinical oncology, Vol. 136, Issue 10, pp. 1573-83, (2010) (PubMed).

Dickey, Redon, Nakamura, Baird, Sedelnikova, Bonner: "H2AX: functional roles and potential applications." dans: Chromosoma, Vol. 118, Issue 6, pp. 683-92, (2009) (PubMed).

Détails sur H2AFX

Antigène: H2AFX (H2A Histone Family, Member X (H2AFX))

Autre désignation: Histone H2A.X (H2AFX Produits)

Synonymes: anticorps H2A.X, anticorps H2A/X, anticorps H2AX, anticorps AW228881, anticorps H2ax, anticorps Hist5-2ax, anticorps gammaH2ax, anticorps zgc:56329, anticorps h2a.x, anticorps h2a/x, anticorps h2ax, anticorps RGD1566119, anticorps h2a, anticorps h2afx, anticorps H2A histone family member X, anticorps H2A histone family, member X, anticorps H2A histone family member X L homeolog, anticorps histone cluster 1, H2ah, anticorps histone cluster 2, H2ab S homeolog, anticorps histone H2AX, anticorps H2AFX, anticorps H2afx, anticorps h2afx, anticorps h2afx.L, anticorps HIST1H2AH, anticorps hist2h2ab.S, anticorps LOC100522201, anticorps LOC100720536

Sujet: Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation

Poids moléculaire: 15 kDa

NCBI Accession: NP_002096

UniProt: P16104

Pathways: Telomere Maintenance, Réparation de l'ADN, Positive Regulation of Response to DNA Damage Stimulus