GCLC anticorps (AA 295-313)

N° du produit ABIN372566

L’anticorps Lapin Polyclonal anti-GCLC a été validé pour WB, IP, IHC (p) et EIA. Il convient pour détecter GCLC dans des échantillons de Humain, Souris, Rat, Boeuf (Vache) et Singe.

Aperçu rapide pour GCLC anticorps (AA 295-313) (ABIN372566)

Antigène: GCLC (Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC))

Reactivité: Humain, Souris, Rat, Boeuf (Vache), Singe

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp GCLC est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Détail du produit anti-GCLC anticorps

Épitope: AA 295-313

Specificité: Recognizes the heavy subunit of GCS.

Réactivité croisée (Details): Species reactivity (expected):Human, Monkey, Mouse and Rat, Bovine (weak).
Species reactivity (tested):Rat.

Purification: Protein A Chromatography.

Immunogène: Synthetic peptide derived from corresponding to aa 295-313 of Rat GCLC (heavy subunit of GCS).

Isotype: IgG

Information d'application

Indications d'application: ELISA. Western Blot. Immunoprecipitation. Immunohistochemistry on Paraffin Sections: 5 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 10 mM PBS, pH 7.4 containing 0.09 % Sodium Azide as presrevtaive and 40 % Glycerol, 0.2 % BSA as stabilizers..

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (Add 40-50% glycerol) at-20 °C for longer.

Détails sur GCLC

Antigène: GCLC (Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC))

Autre désignation: GCLC / GLCLC

Sujet: Glutathione (GSH) plays an important role in detoxification of oxidants and toxins from the cells. In most cells GSH is synthesized de novo in two steps catalysed by Gamma Glutamylcysteine Synthetase (GCS) / Glutamate-cystein Ligase and GSH synthetase. GCS catalyses the first and rate limiting step and is important in GSH homeostasis. It is composed of two subunits: heavy and light. These subunits are coded by different genes which are controlle by different mechanisms. The heavy subunit carries the catalytic activity and can be inhibited through feedback mechanism by GSH. Binding of light subunit to heavy subunit reduces the Michaelis-Menten constant for glutamate to a physiological concentration of 1.2 mM and increases the inhibitory constant for GSH.Synonyms: GCS heavy chain, GLCL, GLCLC, Gamma-ECS, Gamma-glutamylcysteine synthetase, Glutamate-cysteine ligase catalytic subunit

ID gène: 2729

NCBI Accession: NP_001184044

UniProt: P48506

Pathways: Cell RedoxHomeostasis