GLRA1 anticorps (N-Term)

N° du produit ABIN372657

Cet anticorps anti-GLRA1 est un anticorps Lapin Polyclonal détectant GLRA1 dans WB, DB et IHC (fro). Adapté pour Humain, Rat et Souris.

Aperçu rapide pour GLRA1 anticorps (N-Term) (ABIN372657)

Antigène: GLRA1 (Glycine Receptor, alpha 1 (GLRA1))

Reactivité: Humain, Rat, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp GLRA1 est non-conjugé

Application: Western Blotting (WB), Dot Blot (DB), Immunohistochemistry (Frozen Sections) (IHC (fro))

Détail du produit anti-GLRA1 anticorps

Épitope: N-Term

Specificité: This antibody recognizes the ~48k alpha-1 and alpha-2 Subunits of the Glycine Receptor in Western blots of rat spinal cord and brain stem and in cell extracts. Immunolabeling blocked by preadsorption of antibody with the peptide immunogen. Does not recognize other Glycine Receptor subunits.

Réactivité croisée (Details): Species reactivity (tested):Human, Mouse and Rat.

Purification: Affinity Chromatography.

Immunogène: Peptide from the N-terminus region of the alpha-1 subunit of rat Glycine Receptor.

Isotype: IgG

Information d'application

Indications d'application: Dot blot: 1/1000. Western blot: 1/1000. Immunohistochemistry: 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore in 100 μL PBS (137 mM NaCl, 7.5 mM Na2HPO4, 2.7 mM KCl, 1.5 mM KH2PO4, pH 7.4) before use.

Buffer: 5 mM Ammonium Bicarbonate.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: -20 °C

Stockage commentaire: After reconstitution: Store the antibody undiluted (in aliquots) at-20 °C.

Détails sur GLRA1

Antigène: GLRA1 (Glycine Receptor, alpha 1 (GLRA1))

Autre désignation: Glycine Receptor alpha-1

Sujet: Glycine is an important inhibitory transmitter in the brainstem and spinal cord. Glycine receptors are members of the ligand-gated ion channel family (LGICs) that mediate rapid chemical neurotransmission (Schofield et al., 2003). The binding of glycine to its receptor produces a large increase in chloride conductance, which causes membrane hyperpolarization. Glycine receptors are anchored at inhibitory chemical synapses by a cytoplasmic protein, gephyrin (Fischer et al., 2000). The glycine receptor has been used to great advantage in the identification of the binding sites for alcohol on the LGIC family of proteins (Beckstead et al., 2001, Mihic et al., 1997). These receptors have also been extremely useful in studies of synaptic clustering of receptors (Craig and Lichtman, 2001). The glycine receptor may also act in concert with an NMDAR subunit to form an excitatory receptor (Chatterton et al., 2002).Synonyms: GLRA1, Glycine receptor 48 kDa subunit, Glycine receptor strychnine-binding subunit

ID gène: 2741

NCBI Accession: NP_000162

UniProt: P23415

Pathways: Synaptic Membrane