Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))
Specificité
Specific for the ~60k Tyrosine Hydroxylase protein in Western Blots of Rat brain lysates and PC-12 cells stimulated by Okadaic Acid. The antibody cross reacts with all mammalian and at least some non-mammalian forms of the enzyme in Western blots and in IHC/IF.
Réactivité croisée (Details)
Species reactivity (tested):Mammalia.
Purification
Affinity Chromatography.
Immunogène
Native rat tyrosine hydroxylase, purified from pheochromocytoma.
Western Blot/Dot Blot: 1/1,000. Immunofluorescence 1/1000 (Frozen Sections). Immunohistochemistry on Frozen Sections: 1/1000. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Format
Liquid
Buffer
10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.
Conseil sur la manipulation
Avoid repeated freezing and thawing.
Stock
-20 °C
Stockage commentaire
Store the antibody undiluted (in aliquots) at-20 °C.
Tyrosine hydroxylase (TH) is the rate-limiting enzyme in the synthesis of the catecholamines dopamine and norepinephrine. TH antibodies can therefore be used as markers for dopaminergic and noradrenergic neurons in a variety of applications including depression, schizophrenia, Parkinson's disease and drug abuse (Kish et al., 2001, Zhu et al., 2000, Zhu et al., 1999). TH antibodies can also be used to explore basic mechanisms of dopamine and norepinephrine signaling (Witkovsky et al., 2000, Salvatore et al., 2001).Synonyms: TYH, Tyrosine 3-hydroxylase