Fibrinogen anticorps

N° du produit ABIN372857

L’anticorps Mouton Polyclonal anti-Fibrinogen a été validé pour EIA, IF, IHC (p) et IHC (fro). Il convient pour détecter Fibrinogen dans des échantillons de Humain, Souris et Rat.

Aperçu rapide pour Fibrinogen anticorps (ABIN372857)

Antigène: Fibrinogen

Reactivité: Humain, Souris, Rat

Hôte: Mouton

Clonalité: Polyclonal

Conjugué: Cet anticorp Fibrinogen est non-conjugé

Application: Enzyme Immunoassay (EIA), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Détail du produit anti-Fibrinogen anticorps

Réactivité croisée (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Affinity Chromatography on Protein G

Immunogène: Human Fibrinogen purified from plasma

Isotype: IgG

Information d'application

Indications d'application: Immunofluorescence. ELISA: 1/50-1/100. Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin Sections: 5 μg/mL. This Fibrinogen antibody was validated for use in immunohistochemistry on a panel of 21formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigenretrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides wereincubated with biotinylated secondary antibody, followed by alkalinephosphatase-streptavidin and chromogen.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 5.0 mg/mL

Buffer: PBS, pH 7.4, 0.09 % Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Détails sur Fibrinogen

Antigène: Fibrinogen

Sujet: Fibrinogen is the main protein of blood coagulation system. It is a large protein and it consists of two identical subunits that contain three polypeptide chains: alpha, beta and gamma. All chains are connected with each other by a number of disulfide bonds. Fibrinopeptides A (1 to 16 amino acids) and B (1 to 17 amino acids) are released by thrombin from the N terminal parts of alpha and beta chains, respectively. In this way fibrinogen is converted into fibrin, which by means of polymerization forms a fibrin clot. Fibrinogen clotting underlies pathogenesis of MI, thromboembolism and thromboses of arteries and veins, since fibrin is the main substrate for thrombus formation. Fibrinogen activation is also involved in pathogenesis of inflammation, tumor growth and many other diseases. The normal fibrinogen concentration in plasma is about 3 mg/mL. The elevated level of fibrinogen in patient's blood is regarded as an independent risk factor for cardiovascular diseases. An increase in blood fibrinogen concentration was shown to be a strong predictor of coronary heart disease (Sonel A. et al, and Rapold H.J. et al). All these facts make fibrinogen an important parameter in the diagnosis of cardiovascular diseases.Synonyms: FGA, FGB, FGG