CDKN1B anticorps (C-Term)

N° du produit ABIN374083

Cet anticorps Chèvre Polyclonal détecte spécifiquement CDKN1B dans WB et EIA. Il présente une réactivité avec des échantillons de Humain, Souris, Rat, Chien et Boeuf (Vache).

Aperçu rapide pour CDKN1B anticorps (C-Term) (ABIN374083)

Antigène: CDKN1B (Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B))

Reactivité: Humain, Souris, Rat, Chien, Boeuf (Vache)

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp CDKN1B est non-conjugé

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Détail du produit anti-CDKN1B anticorps

Épitope: C-Term

Séquence: C-EQTPKKPGLR RRQT

Specificité: This antibody detects P27Kip1 / CDKN1B at C-term.

Réactivité croisée (Details): Species reactivity (expected):Mouse, Rat, Cow, Canine.
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogène: Peptide from C Terminus of the protein sequence according to NP_004055.1 Genename: CDKN1B

Information d'application

Indications d'application: Peptide ELISA: Limit dilution 1: 32000. Western blot: 1-3 μg/mL. Approx 26 kDa band observed in Human Breast and Prostatelysates (calculated MW of 22.6 kDa according to NP_004055.1). The observed molecularweight corresponds to earlier findings in literature with different antibodies (Xia et al, ProcNatl Acad Sci U S A.2005 Sep 27,102(39): 14028-33, PMID: 16169901).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur CDKN1B

Antigène: CDKN1B (Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B))

Autre désignation: CDKN1B / KIP1

Sujet: Cell cycle progression is regulated by cyclins and their cognate Cdks. p27 KIP 1 is a cell cycle regulatory mitotic inhibitor of cdk activity. p27 KIP 1 is a candidate tumor suppressor gene, and has been proposed to function as a possible mediator of TGF beta induced G1 arrest. p27 KIP 1 is up regulated in response to antimitogenic stimuli. The increased protein expression of p27 results in cellular arrest by binding to cyclin/Cdk complexes such as cyclin D1/Cdk4. p27 Kip1 is regulated by phosphorylation on serine 10 (S10) and threonine 187 (T187). Phosphorylation by CDK2 on T187 results in ubiquitylation and degradation of p27 Kip 1, while phosphorylation by hKIS on S10 signals the nuclear export to the cytoplasm.Synonyms: Cyclin-dependent kinase inhibitor 1B, Cyclin-dependent kinase inhibitor p27, p27Kip1

ID gène: 1027

NCBI Accession: NP_004055

UniProt: P46527

Pathways: Cycle Cellulaire, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Sensory Perception of Sound, Mitotic G1-G1/S Phases, DNA Replication, Positive Regulation of Endopeptidase Activity, Synthesis of DNA, Autophagy