KPNA4 anticorps (C-Term)

N° du produit ABIN374394

Cet anticorps anti-KPNA4 est un anticorps Chèvre Polyclonal détectant KPNA4 dans WB, IHC (p) et EIA. Adapté pour Humain, Souris, Rat, Boeuf (Vache) et Chien.

Aperçu rapide pour KPNA4 anticorps (C-Term) (ABIN374394)

Antigène: KPNA4 (Karyopherin (Importin) alpha 4 (KPNA4))

Reactivité: Humain, Souris, Rat, Boeuf (Vache), Chien

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp KPNA4 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Détail du produit anti-KPNA4 anticorps

Épitope: C-Term

Séquence: C-NSSANVPTEG FQF

Specificité: This antibody detects Importin subunit alpha-4 at C-term.

Réactivité croisée (Details): Species reactivity (expected):Rat, Canine (Dog), Cow.
Species reactivity (tested):Human, Mouse.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.

Immunogène: Peptide from C Terminus of the protein sequence according to NP_002259.1 Genename: KPNA4

Information d'application

Indications d'application: Peptide ELISA: Limit dilution 1: 32000. Western blot: 0.2-1 μg/mL. Approx 55-60 kDa band observed in Human Testis and Ovarylysates (predicted size of approx.50 kDa according to NP_002259.1). Immunohistochemistry: In paraffin embedded Human Testis, shows strong nuclearstaining of some cells in the seminiferous tubules (3 μg/mL). In paraffin embedded HumanSmall Intestine shows pixulate staining in nuclei of enterocytes (1-2 μg/mL).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur KPNA4

Antigène: KPNA4 (Karyopherin (Importin) alpha 4 (KPNA4))

Autre désignation: KPNA4 / Importin alpha-4

Sujet: KPNA4 (karyopherin alpha 4), also known as importin alpha 3, is a member of the importin alpha family of proteins which includes KPNA1 (importin alpha 5), KPNA2 (importin alpha 1), KPNA3 (importin alpha 4), KPNA4 (importin alpha 3), KPNA5 (importin alpha 6), and KPNA6 (importin alpha 7). The importin alpha family of proteins plays a role in the import of proteins from the cytoplasm to the nucleus. The import of proteins from the cytoplasm to the nucleus involves docking the protein to receptors associated with the nuclear pore complex (NPC) followed by translocation through the nuclear pore. Importin alpha proteins bind the nuclear localization signal (NLS) of cargo proteins and function as adaptor proteins for the nuclear pore receptor KPNB1 (importin beta 1). KPNB1 is responsible for docking the importin-cargo complex to the cytoplasmic side of the NPC for its translocation through the NPC. KPNA3 (Importin alpha 4) and KPNA4 (importin alpha 3) have been reported to mediate the nuclear import of TNF-alpha induced nuclear import of NF-kappaB p50/p65 heterodimers. The nuclear import of karyophilic proteins is directed by short amino acid sequences termed nuclear localization signals (NLSs). Karyopherins, or importins, are cytoplasmic proteins that recognize NLSs and dock NLS-containing proteins to the nuclear pore complex. The protein encoded by this gene shares the sequence similarity with Xenopus importin-alpha and Saccharomyces cerevisiae Srp1. This protein is found to interact with the NLSs of DNA helicase Q1 and SV40 T antigen.Synonyms: Importin alpha Q1, Karyopherin subunit alpha-4, QIP1

ID gène: 3840

NCBI Accession: NP_002259

UniProt: O00629

Pathways: Protein targeting to Nucleus