VPS35 anticorps (C-Term)

N° du produit ABIN374403

Cet anticorps anti-VPS35 Polyclonal Chèvre (ABIN374403) détecte spécifiquement VPS35 dans WB, IF, IHC (p) et EIA. L’anticorps est réactif avec des échantillons de Humain, Souris, Rat et Boeuf (Vache).

Aperçu rapide pour VPS35 anticorps (C-Term) (ABIN374403)

Antigène: VPS35 (Vacuolar Protein Sorting 35 (VPS35))

Reactivité: Humain, Souris, Rat, Boeuf (Vache)

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp VPS35 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Détail du produit anti-VPS35 anticorps

Épitope: C-Term

Séquence: C-SPESEGPIYE GLIL

Specificité: This antibody detects VPS35 / MEM3 at C-term.

Réactivité croisée (Details): Species reactivity (expected):Mouse, Rat, Cow.
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogène: Syntehtic peptide from the C-Terminus of Human VPS35 (NP_060676.2). Genename: VPS35

Information d'application

Indications d'application: Peptide ELISA: 1/16000. Western blot: Approx 90 kDa band observed in Human Brain and Testis lysates (predictedMW of 90 kDa according to NP_060676.2). Recommended concentration: 1 - 3 μg/mL. Immunohistochemistry on paraffin sections: Human Testis, shows positive staining inLeydig cells, and cytoplasmic staining (dot-like) of some epithelial cells of the epididymisand of the rete testis. Immunoflurescence: Staining of VPS35 (red) in human melanoma cells 1205LU: Please seeScott KL et al. , Nature.2009 Jun 25,459(7250): 1085-90. , PMID: 19553991.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur VPS35

Antigène: VPS35 (Vacuolar Protein Sorting 35 (VPS35))

Autre désignation: VPS35 / MEM3

Sujet: Synonyms: Maternal-embryonic 3, TCCCTA00141, Vacuolar protein sorting-associated protein 35, Vesicle protein sorting 35

ID gène: 55737

NCBI Accession: NP_060676

UniProt: Q96QK1