BAF53A anticorps (C-Term)

N° du produit ABIN374427

Cet anticorps anti-BAF53A Polyclonal Chèvre (ABIN374427) détecte spécifiquement BAF53A dans WB. L’anticorps est réactif avec des échantillons de Humain, Souris, Rat, Boeuf (Vache) et Chien.

Aperçu rapide pour BAF53A anticorps (C-Term) (ABIN374427)

Antigène: BAF53A (Actin-Like 6A (BAF53A))

Reactivité: Humain, Souris, Rat, Boeuf (Vache), Chien

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp BAF53A est non-conjugé

Application: Western Blotting (WB)

Détail du produit anti-BAF53A anticorps

Épitope: C-Term

Séquence: YEEGGKQCVE RKCP

Specificité: This antibody detects ACTL6A / BAF53 at C-term.

Réactivité croisée (Details): Species reactivity (expected):Mouse, Rat, Dog, CowSpecies reactivity (tested):Human

Purification: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogène: Peptide from C Terminus of the protein sequence according to NP_004292.1, NP_817126.1, NP_057272.1. Genename: ACTL6A / ACTL6B

Information d'application

Indications d'application: Peptide ELISA: Limit dilution 1: 64000. Western Blot: 1-3 μg/mL. Approx 48 kDa band observed in Hela lysates (predicted MW of48 kDa according to NP_057272, NP_004292).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.

Détails sur BAF53A

Antigène: BAF53A (Actin-Like 6A (BAF53A))

Autre désignation: ACTL6A / BAF53

Sujet: ACTL6A is a family member of actin-related proteins (ARPs), which share significant amino acid sequence identity to conventional actins. Both actins and ARPs have an actin fold, which is an ATP-binding cleft, as a common feature. The ARPs are involved in diverse cellular processes, including vesicular transport, spindle orientation, nuclear migration and chromatin remodeling. ACTL6A is a 53 kDa subunit of the BAF (BRG1/brm-associated factor) complex in mammals, which is functionally related to SWI/SNF complex in S. cerevisiae and Drosophila, the latter is thought to facilitate transcriptional activation of specific genes by antagonizing chromatin-mediated transcriptional repression. Together with beta-actin, it is required for maximal ATPase activity of BRG1, and for the association of the BAF complex with chromatin/matrix. It is required for maximal ATPase activity of SMARCA4/BRG1 and for association of the SMARCA4/BRG1 containing remodelling complex BAF with chromatin/nuclear matrix. ACTL6A is a component of the NuA4 histone acetyltransferase (HAT) complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histone H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage.Synonyms: 53 kDa BRG1-associated factor A, Actin-like protein 6A, Actin-related protein Baf53a, ArpNbeta, BAF53A, INO80 complex subunit K, INO80K

ID gène: 86

NCBI Accession: NP_004292

UniProt: O96019

Pathways: Chromatin Binding, Photoperiodism