LIME anticorps (C-Term)

N° du produit ABIN374438

L’anticorps anti-LIME Polyclonal Chèvre est utilisé pour la détection de LIME dans des échantillons de Souris et Rat. Il a été validé pour WB.

Aperçu rapide pour LIME anticorps (C-Term) (ABIN374438)

Antigène: LIME (LIME1) (Lck Interacting Transmembrane Adaptor 1 (LIME1))

Reactivité: Souris, Rat

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp LIME est non-conjugé

Application: Western Blotting (WB)

Détail du produit anti-LIME anticorps

Épitope: C-Term

Séquence: C-ENVYESIKEM GL

Specificité: This antibody recognizes LIME1.

Réactivité croisée (Details): Species reactivity (expected):Rat.
Species reactivity (tested):Mouse

Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.

Immunogène: Peptide from the C Terminus of the protein sequence according to NP_076173.1.

Information d'application

Indications d'application: Peptide ELISA: 1/128000. Western Blot: 0.5-2.0 μg/mL, Approx 32 kDa band observed in Mouse Heart lysates(predicted MW of 32 kDa according to NP_076173.1).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur LIME

Antigène: LIME (LIME1) (Lck Interacting Transmembrane Adaptor 1 (LIME1))

Autre désignation: LIME1

Sujet: LIME (Lck-interacting molecule) is a new raft-associated adaptor protein involved in regulation of T cell activation by coreceptors. LIME becomes tyrosine phosphorylated after cross-linking of the CD4 or CD8 coreceptors. Phospho-LIME associates with the Src family kinase Lck and its negative regulator, Csk. Ectopic expression of LIME in Jurkat T cells results in an increase of Csk in lipid rafts, increased phosphorylation of Lck and higher Ca2+ response to CD3 stimulation.Synonyms: LIME, LIME-1, LIME1, Lck-interacting membrane protein, Lck-interacting molecule, Lck-interacting transmembrane adapter 1

ID gène: 54923

NCBI Accession: NP_060276

UniProt: Q9H400