PD-L1 anticorps (C-Term)

N° du produit ABIN374512

Cet anticorps Chèvre Polyclonal détecte spécifiquement PD-L1 dans WB et IHC (p). Il présente une réactivité avec des échantillons de Humain.

Aperçu rapide pour PD-L1 anticorps (C-Term) (ABIN374512)

Antigène: PD-L1 (CD274 (PD-L1))

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp PD-L1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-PD-L1 anticorps

Épitope: C-Term

Séquence: CKKQSDTHLE ET

Specificité: This antibody recognizes Human CD274 / PDL1. Other species not tested.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogène: Synthetic peptide from the C-Terminus of Human (NP_054862.1) Genename: CD274

Information d'application

Indications d'application: Peptide ELISA: 1/8000. Western blot: 0.3-1.0 μg/mL. Approx 35 kDa band observed in Human Heart lysates(calculated MW of 33.5 kDa according to NP_054862.1). Immunohistochemistry: 4-6 μg/mL. In paraffin embedded Human Skeletal Muscle shows staining of the striated muscularfibres.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C.

Détails sur PD-L1

Antigène: PD-L1 (CD274 (PD-L1))

Autre désignation: CD274 / PDL1

Sujet: CD274 [PDL1] is expressed by antigen presenting cells, including human peripheral blood monocytes stimulated with interferon gamma, and activated human and murine dendritic cells. In addition, PDL1 is expressed in nonlymphoid tissues such as heart and lung. The relative levels of inhibitory PDL1 and costimulatory B7 1/B7 2 signals on antigen presenting cells may determine the extent of T cell activation and consequently the threshold between tolerance and autoimmunity. PDL1 expression on nonlymphoid tissues and its potential interaction with PD1 may subsequently determine the extent of immune responses at sites of inflammation. PDL1 is thought to be involved in fetomaternal tolerance.Synonyms: B7 homolog 1, B7H1, PD-L1, PDCD1 ligand 1, PDCD1L1, PDCD1LG1, Programmed cell death 1 ligand 1, Programmed death ligand 1

ID gène: 29126, 9606

UniProt: Q9NZQ7

Pathways: Cancer Immune Checkpoints