Complement Factor H anticorps (Internal Region)

N° du produit ABIN374588

L’anticorps anti-Complement Factor H Polyclonal Chèvre est utilisé pour la détection de Complement Factor H dans des échantillons de Humain. Il a été validé pour WB.

Aperçu rapide pour Complement Factor H anticorps (Internal Region) (ABIN374588)

Antigène: Complement Factor H (CFH)

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp Complement Factor H est non-conjugé

Application: Western Blotting (WB)

Détail du produit anti-Complement Factor H anticorps

Épitope: Internal Region

Specificité: This antibody is expected to recognize isoform a of Complement factor H (CFH) only.

Purification: Affinity chromatography

Immunogène: Peptide with sequence C-HLVPDRKKDQYK, from the internal region of the protein sequence

Information d'application

Indications d'application: Peptide ELISA: 1/32000. Western Blot: 0.03 - 0.1 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0,5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur Complement Factor H

Antigène: Complement Factor H (CFH)

Sujet: The complement Factor H protein is secreted into the bloodstream and acts in the regulation of complement activation. Mutations leading to changes in this protein have been linked with HUS (hemolytic-uremic syndrome) and chronic hypocomplementemic nephropathy. Factor H is mainly synthesised in the liver but also in macrophages and endothelium. It is primarily aplasma glycoprotein but is also found in platelets and there is a membrane bound form on some leukocytes. Consisting of a single polypeptide, the major form of Factor H has a molecular weight of 155 kDa. There are two truncated forms, a non-glycosylated 49 kDa form and a glycosylated 39-43 kDaform. Plasma concentrations are in the range 200-600 mg/L for the 155 kDa form and 1-5 mg/L for thetruncated forms. Factor H is a major regulatory protein of the complement system. By binding to C3b it either displacesor prevents the binding of Bb (activated Factor B). When bound to Factor H, C3b is susceptible tocleavage by Factor 1 to yield iC3b. Factor H is released or modified following this cleavage. The regulatory role of Factor H is essential because C3bBb is not only a C5 convertase but a C3 convertaseand so has a positive feedback effect, potentially consuming the entire C3 pool if unregulated.Synonyms: CFH, H factor 1, HF, HF1, HF2

ID gène: 3075

NCBI Accession: NP_000177

UniProt: P08603

Pathways: Système du Complément, Cellular Response to Molecule of Bacterial Origin