14-3-3 sigma/SFN anticorps (Internal Region, N-Term)

N° du produit ABIN374638

Cet anticorps Chèvre Polyclonal détecte spécifiquement 14-3-3 sigma/SFN dans WB et IHC (p). Il présente une réactivité avec des échantillons de Humain, Souris, Rat et Chien.

Aperçu rapide pour 14-3-3 sigma/SFN anticorps (Internal Region, N-Term) (ABIN374638)

Antigène: 14-3-3 sigma/SFN (SFN) (Stratifin (SFN))

Reactivité: Humain, Souris, Rat, Chien

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp 14-3-3 sigma/SFN est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-14-3-3 sigma/SFN anticorps

Épitope: Internal Region, N-Term

Séquence: C-DLHTLSEDSY KDST

Specificité: This antibody recognizes 4-3-3 sigma/Stratifin.

Réactivité croisée (Details): Species reactivity (expected):Mouse, Rat, Canine (Dog) and Porcine.
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.

Immunogène: Peptide with sequence from the internal region of the protein sequence according to NP_006133.1.

Information d'application

Indications d'application: Peptide ELISA: 1/64000. Western Blot: 0.1-0.3 μg/mL. Approx.28 kDa band observed in Human Skin lysates (calculated MW of 27.8 kDa accordingto NP_006133.1). Immunohistochemistry on Paraffin Sections: 2-3 μg/mL. In paraffin embedded Human Skin, this antibody shows strong nuclear staining in selectedcells of the basal layer.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur 14-3-3 sigma/SFN

Antigène: 14-3-3 sigma/SFN (SFN) (Stratifin (SFN))

Autre désignation: 14-3-3 Protein sigma / SFN

Sujet: Members of the 14-3-3 family of proteins are highly conserved proteins, localized in neurons, and are axonally transported to the nerve terminals. They are also present, at lower levels, in various other eukaryotic tissues. 14-3-3 proteins appear to play important roles in a variety of signal transduction pathways, including those involved in cell cycle regulation and cell survival. As 14-3-3 proteins bind to specific phosphoserine-containing sequences they are likely to have an important role in signaling pathways mediated by serine/threonine protein kinases. 14-3-3 sigma may be required for Raf-1 kinase activity and phosphorylation, among many other functions.Synonyms: Epithelial cell marker protein 1, HME1, Stratifin

ID gène: 2810, 9606

UniProt: P31947

Pathways: Signalisation p53, Myometrial Relaxation and Contraction