FZD7 anticorps (C-Term)

N° du produit ABIN374921

L’anticorps anti-FZD7 Polyclonal Chèvre est utilisé pour la détection de FZD7 dans des échantillons de Humain et Souris. Il a été validé pour WB.

Aperçu rapide pour FZD7 anticorps (C-Term) (ABIN374921)

Antigène: FZD7 (Frizzled Family Receptor 7 (FZD7))

Reactivité: Humain, Souris

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp FZD7 est non-conjugé

Application: Western Blotting (WB)

Détail du produit anti-FZD7 anticorps

Épitope: C-Term

Séquence: C-RFYHRLSHSS KGET

Specificité: This antibody recognizes Frizzled 7 (C-term).

Réactivité croisée (Details): Species reactivity (expected):Mouse.
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.

Immunogène: Peptide with sequence C-RFYHRLSHSSKGET from the C Terminus of Human FZD7 (NP_003498.1).

Information d'application

Indications d'application: Peptide ELISA: Detection Limit Dilution: 1/64000. Western Blot: 0.3-1 μg/mL. Approx 60 kDa band observed in lysates of cell lines HEK293 andHepG2 (calculated MW of 63.0 kDa according to NP_003498.1). Immunohistochemistry on Paraffin Sections: 5 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur FZD7

Antigène: FZD7 (Frizzled Family Receptor 7 (FZD7))

Autre désignation: FZD7 / Frizzled-7

Sujet: Members of the 'frizzled' gene family encode 7-transmembrane domain proteins that are receptors for Wnt signaling proteins. The Frizzled 7 protein contains an N-terminal signal sequence, 10 cysteine residues typical of the cysteine-rich extracellular domain of Frizzled family members, 7 putative transmembrane domains, and an intracellular C-terminal tail with a PDZ domain-binding motif. Frizzled 7 gene expression may downregulate APC function and enhance beta-catenin-mediated signals in poorly differentiated human esophageal carcinomas. Frizzled 7 expression has been reported in brain, gastrointestinal tract, heart, fetal kidney, fetal lung, placenta, skeletal muscle, and various cancers. ESTs have been isolated from a wide variety of normal and cancer libraries.Synonyms: Fz-7, mFz7

ID gène: 8324

NCBI Accession: NP_003498

UniProt: O75084

Pathways: Signalisation WNT, Stem Cell Maintenance