XBP1
Reactivité: Souris
WB
Hôte: Lapin
Polyclonal
RB31994
unconjugated
Indications d'application
Peptide ELISA: Detection Limit Dilution: 1/4000. Western Blot: 1-3 μg/mL. Approx 38 kDa band observed in Nuclear lysates of cell line HeLa(calculated MW of 40.8 kDa according to NP_001073007.1). Immunohistochemistry on Paraffin Sections: 5 μg/mL. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
0.5 mg/mL
Buffer
Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation
Avoid repeated freezing and thawing.
Stock
4 °C/-20 °C
Stockage commentaire
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Yokouchi, Hiramatsu, Hayakawa, Okamura, Du, Kasai, Takano, Shitamura, Shimada, Yao, Kitamura: "Involvement of selective reactive oxygen species upstream of proapoptotic branches of unfolded protein response." dans: The Journal of biological chemistry, Vol. 283, Issue 7, pp. 4252-60, (2008) (PubMed).
This gene encodes a transcription factor that regulates MHC class II genes by binding to a promoter element referred to as an X box. The protein contains two domains commonly found in transcription factors that confer DNA binding and dimerization capability. This gene product was also separately identified as a cellular transcription factor that binds to an enhancer in the promoter of the T cell leukemia virus type 1 promoter. It may increase expression of viral proteins by acting as the DNA binding partner of a viral transactivator. There is also a recent finding that the same agents that cause an ER stress response, the unfolded protein response, also increase transcription of this gene. This increase in transcription requires an ER stress response consensus binding element in the promoter.Synonyms: Tax-responsive element-binding protein 5, X-box-binding protein 1, XBP-1, XBP2