XBP1 anticorps (Internal Region)

N° du produit ABIN374982

Cité dans 1 publication. Cet anticorps anti-XBP1 Polyclonal Chèvre (ABIN374982) détecte spécifiquement XBP1 dans WB, IHC (p) et EIA. L’anticorps est réactif avec des échantillons de Humain et Chien.

Aperçu rapide pour XBP1 anticorps (Internal Region) (ABIN374982)

Antigène: XBP1 (X-Box Binding Protein 1 (XBP1))

Reactivité: Humain, Chien

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp XBP1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Détail du produit anti-XBP1 anticorps

Épitope: Internal Region

Séquence: C-NHSWEDTFAN ELFPQ

Specificité: This antibody is expected to recognize isoform XBP1(S) only.

Réactivité croisée (Details): Species reactivity (expected):Canine.
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.

Immunogène: Synthetic peptide from an Internal region of Human XBP1 (NP_001073007.1) Genename: XBP1

Information d'application

Indications d'application: Peptide ELISA: Detection Limit Dilution: 1/4000. Western Blot: 1-3 μg/mL. Approx 38 kDa band observed in Nuclear lysates of cell line HeLa(calculated MW of 40.8 kDa according to NP_001073007.1). Immunohistochemistry on Paraffin Sections: 5 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Références pour anticorps anti-XBP1 (ABIN374982)

Yokouchi, Hiramatsu, Hayakawa, Okamura, Du, Kasai, Takano, Shitamura, Shimada, Yao, Kitamura: "Involvement of selective reactive oxygen species upstream of proapoptotic branches of unfolded protein response." dans: The Journal of biological chemistry, Vol. 283, Issue 7, pp. 4252-60, (2008) (PubMed).

Détails sur XBP1

Antigène: XBP1 (X-Box Binding Protein 1 (XBP1))

Autre désignation: XBP1 / TREB5

Sujet: This gene encodes a transcription factor that regulates MHC class II genes by binding to a promoter element referred to as an X box. The protein contains two domains commonly found in transcription factors that confer DNA binding and dimerization capability. This gene product was also separately identified as a cellular transcription factor that binds to an enhancer in the promoter of the T cell leukemia virus type 1 promoter. It may increase expression of viral proteins by acting as the DNA binding partner of a viral transactivator. There is also a recent finding that the same agents that cause an ER stress response, the unfolded protein response, also increase transcription of this gene. This increase in transcription requires an ER stress response consensus binding element in the promoter.Synonyms: Tax-responsive element-binding protein 5, X-box-binding protein 1, XBP-1, XBP2

ID gène: 7494, 9606

UniProt: P17861

Pathways: ER-Nucleus Signaling, Unfolded Protein Response