Clusterin anticorps (C-Term)

N° du produit ABIN375042

Cité dans 1 publication. Cet anticorps anti-Clusterin Polyclonal Chèvre (ABIN375042) détecte spécifiquement Clusterin dans WB et EIA. L’anticorps est réactif avec des échantillons de Rat et Souris.

Aperçu rapide pour Clusterin anticorps (C-Term) (ABIN375042)

Antigène: Clusterin (CLU)

Reactivité: Rat, Souris

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp Clusterin est non-conjugé

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Détail du produit anti-Clusterin anticorps

Épitope: C-Term

Séquence: C-EKALQEYRRK SRAE

Specificité: This antibody recognizes Clusterin / ApoJ (C-term).

Réactivité croisée (Details): Species reactivity (tested):Mouse, Rat.

Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide

Immunogène: Peptide with sequence from the C Terminus of the protein sequence according to NP_038520.1. Genename: Clu

Information d'application

Indications d'application: Peptide ELISA: 1/8000. Western Blot: 0.3-1 μg/mL. Approx.37 kDa band observed in Mouse Brain and Testis lysatesand in Rat Brain and Ovary lysates (calculated MW of 51.7 kDa according to Mouse

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Tris buffered saline, pH ~7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Références pour anticorps anti-Clusterin (ABIN375042)

Charnay, Imhof, Vallet, Hakkoum, Lathuiliere, Poku, Aronow, Kovari, Bouras, Giannakopoulos: "Clusterin expression during fetal and postnatal CNS development in mouse." dans: Neuroscience, Vol. 155, Issue 3, pp. 714-24, (2008) (PubMed).

Détails sur Clusterin

Antigène: Clusterin (CLU)

Autre désignation: Apolipoprotein J (Apo J)

Sujet: At least 9 distinct polymorphic forms of apolipoproteins are known. The apolipoproteins act as stabilizers of the intact lipoprotein particles. Quantitative measurements of HDL, LDL and VLDL particles in human serum are often used to estimate an individual's relative risk of coronary heart disease. In addition, quantitative immunological measurements of certain apolipoproteins (especially A-1 and B) have been suggested to be more accurate estimators of coronary heart disease than measurements of lipoprotein particles (especially HDL and LDL). Apolipoprotein J is the major secreted product of Sertoli cells and is thought to play a critical role in spermatogenesis. The protein was shown to be a normal constituent of human blood and is also thought to be a control mechanism of the complement cascade. It prevents the binding of a C5b-C7 complex to the membrane of the target cell and in this way inhibits complement-mediated cytolysis. Apolipoprotein J is overexpressed in human prostate and breast cancers and in squamous cell carcinomas. Suppression of Apolipoprotein J renders these cells sensitive to chemotherapeutic drug-mediated apoptosis. It has been proposed that elevated levels of Apolipoprotein J may promote oncogenic transformation and tumor progression by interfering with BAX proapoptotic activities.Synonyms: 40, 40, AAG4, APOJ, Aging-associated gene 4 protein, CLI, Clusterin, Complement cytolysis inhibitor, Complement-associated protein SP-40, KUB1, Ku70-binding protein 1, NA1/NA2, SP-40, Testosterone-repressed prostate message 2

ID gène: 12759, 10090

UniProt: Q06890

Pathways: Apoptose, Negative Regulation of intrinsic apoptotic Signaling