This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Immunogène
This CD73 (NT5E) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human CD73 (NT5E).
NT5E
Reactivité: Humain, Souris, Rat
IHC
Hôte: Lapin
Polyclonal
unconjugated
Indications d'application
IHC-P: 1:10~50
Restrictions
For Research Use only
Format
Liquid
Buffer
Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock
4 °C,-20 °C
Stockage commentaire
Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Date de péremption
6 months
Kim, Piao, Pak, Chung, Han, Hong, Jun, Shim, Choi, Kim: "Umbilical cord mesenchymal stromal cells affected by gestational diabetes mellitus display premature aging and mitochondrial dysfunction." dans: Stem cells and development, Vol. 24, Issue 5, pp. 575-86, (2015) (PubMed).
Doherty, Bai, Hanidziar, Longhi, Lawlor, Putheti, Csizmadia, Nowak, Cheifetz, Moss, Robson: "CD73 is a phenotypic marker of effector memory Th17 cells in inflammatory bowel disease." dans: European journal of immunology, Vol. 42, Issue 11, pp. 3062-72, (2012) (PubMed).
Andrade, Lopez, Noronha, Wink, Borojevic, Margis, Lenz, Battastini, Guma: "Ecto-5'-nucleotidase/CD73 knockdown increases cell migration and mRNA level of collagen I in a hepatic stellate cell line." dans: Cell and tissue research, Vol. 344, Issue 2, pp. 279-86, (2011) (PubMed).
Nadri, Soleimani, Mobarra, Amini: "Expression of dopamine-associated genes on conjunctiva stromal-derived human mesenchymal stem cells." dans: Biochemical and biophysical research communications, Vol. 377, Issue 2, pp. 423-8, (2008) (PubMed).
Ecto-5-prime-nucleotidase catalyzes conversion at neutral pH of purine 5-prime mononucleotides to nucleosides. The enzyme consists of a dimer of 2 identical 70 kD subunits tethered by a glycosyl phosphatidyl inositol moeity to the exterior plasma membrane surface. The enzyme, a marker of lymphocyte differentiation is associated in deficiency with a variety of immunodeficiency diseases. The preferred substrate is AMP. The NT5 gene has been localized to 6q14-q21 by immunofluorescence and a study of a panel of human x mouse hybrids that contained fragments of chromosome 6 as translocations.