MGMT anticorps (C-Term)

N° du produit ABIN391514

Il existe 2+ publications pour ce produit. L’anticorps anti-MGMT Polyclonal Lapin est utilisé pour la détection de MGMT dans des échantillons de Humain. Il a été validé pour WB, FACS et IHC (p).

Aperçu rapide pour MGMT anticorps (C-Term) (ABIN391514)

Antigène: MGMT (O6-Methylguanine-DNA-Methyltransferase (MGMT))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp MGMT est non-conjugé

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: RB19082

Détail du produit anti-MGMT anticorps

Épitope: AA 156-182, C-Term

Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogène: This MGMT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 156-182 amino acids from the C-terminal region of human MGMT.

Isotype: Ig Fraction

Information d'application

Indications d'application: WB: 1:1000. IHC-P: 1:50~100. FC: 1:10~50

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Date de péremption: 6 months

Références pour anticorps anti-MGMT (ABIN391514)

Ge, Guo, Zhang, Lin, Li, Liu, Kong, Fang, Zhao: "Impact of O6-methylguanine-DNA methyltransferase expression on the drug resistance of clear cell renal cell carcinoma." dans: Japanese journal of clinical oncology, (2015) (PubMed).

Wu, Cai, Lin, Xiong, Lu, Mao, Yao, Zhou: "High-mobility group box 2 is associated with prognosis of glioblastoma by promoting cell viability, invasion, and chemotherapeutic resistance." dans: Neuro-oncology, Vol. 15, Issue 9, pp. 1264-75, (2013) (PubMed).

Détails sur MGMT

Antigène: MGMT (O6-Methylguanine-DNA-Methyltransferase (MGMT))

Autre désignation: MGMT

Sujet: MGMT is involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. This protein repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the protein is irreversibly inactivated.

Poids moléculaire: 21646

ID gène: 4255

UniProt: P16455

Pathways: Réparation de l'ADN, Positive Regulation of Response to DNA Damage Stimulus