Mesothelin anticorps (Extracellular Domain)

N° du produit ABIN400817

L’anticorps anti-Mesothelin Monoclonal Souris est utilisé pour la détection de Mesothelin dans des échantillons de Humain. Il a été validé pour WB, IP, IHC (p) et EIA.

Aperçu rapide pour Mesothelin anticorps (Extracellular Domain) (ABIN400817)

Antigène: Mesothelin (MSLN)

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Mesothelin est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Clone: MN-1

Détail du produit anti-Mesothelin anticorps

Épitope: Extracellular Domain

Specificité: This antibody is directed against human mesothelin protein.

Réactivité croisée (Details): Species reactivity (tested):Human.

Attributs du produit: Synonyms: MSLN, MPF, CAK1 antigen, Pre-pro-megakaryocyte-potentiating factor

Purification: Protein A Chromatography.

Immunogène: Recombinant protein corresponding to the extracellular domain of Human Mesothelin

Information d'application

Indications d'application: ELISA: 1/10,000-1/50,000. Immunoprecipitaion. Western blot: 1/1,000. Expect a band approximately 40 kDa in size corresponding to mature mesothelin in theappropriate cell lysate or extract. Immunohistochemistry on Paraffin Sections: 1/100. Archival PEFF human tissues were deparaffinized followed by hydration. Antigen-retrievalis recommended. Block tissues with 1 % BSA in PBS for 30 min at 23 °C. Antibodies are diluted in 1 % BSA andreacted with tissue for 60 min at room temperature.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide as preservative.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C or below forlonger. Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf life: one year from despatch.

Date de péremption: 12 months

Détails sur Mesothelin

Antigène: Mesothelin (MSLN)

Autre désignation: Mesothelin

Sujet: Mesothelin is a glycosyl-phosphatidylinositol-anchored glycoprotein present on the cell surface of various human solid tumors. The mesothelin (MSLN) gene encodes a 71- kDa precursor protein that is processed to a 40- kDa glycosylphosphatidylinositol-anchored protein that composes the mature portion and an NH2-terminal 31- kDa fragment called megakaryocyte-potentiating factor that is released from the cell. Mesothelin is a tumor differentiation antigen present at low levels on a restricted set of normal adult tissues, such as mesothelium, but aberrantly over expressed in mesotheliomas, ovarian, and pancreatic cancers. The biological functions of mesothelin remain elusive. A recent study showed that mesothelin binds to MUC16/CA125, and that this interaction mediates cell adhesion, suggesting that there may be an important role for MUC16/CA125 and mesothelin in the metastatic spread of ovarian cancer.Synonyms: CAK1 antigen, MPF, MSLN, Pre-pro-megakaryocyte-potentiating factor

ID gène: 10232

UniProt: Q13421

Pathways: EGFR Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Carbohydrate Homeostasis, cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process