CD3 anticorps (Activation Site) (PE)

N° du produit ABIN400887

Cet anticorps anti-CD3 est un anticorps Souris Monoclonal détectant CD3 dans FACS. Adapté pour Humain et Souris.

Aperçu rapide pour CD3 anticorps (Activation Site) (PE) (ABIN400887)

Antigène: CD3

Reactivité: Humain, Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD3 est conjugé à/à la PE

Application: Flow Cytometry (FACS)

Clone: APA1-1

Détail du produit anti-CD3 anticorps (PE)

Épitope: Activation Site

Specificité: The antibody recognizes an activation-dependent intracellular epitope of CD3 epsilon. Exposure of the epitope precedes CD3 phosphorylation and recruitment and activation of ZAP70, which initiates the signaling cascade produced by T-cell activation. APA1/1 provides the earliest known marker for TCR-mediated T cell activation.

Réactivité croisée (Details): Species reactivity (tested):Human, Mouse

Attributs du produit: Synonyms: T3/Leu-4, T-cell surface glycoprotein CD3, T-cell surface antigen T3/Leu-4

Immunogène: Purified human CD3 proteins isolated from thymus

Isotype: IgG1

Information d'application

Indications d'application: Flow Cytometry: 5 μg/mL (starting point).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.1 mg/mL

Buffer: Phosphate buffered saline (PBS) containing 15 mM sodium azide and 0.2 % (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store the antibody at 2 - 8 °C. DO NOT FREEZE! This product is photosensitive and should be protected from light.
Shelf life: one year from despatch.

Date de péremption: 12 months

Détails sur CD3

Antigène: CD3

Autre désignation: CD3

Sujet: CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation.Synonyms: T-cell surface antigen T3/Leu-4, T-cell surface glycoprotein CD3, T3/Leu-4

ID gène: 916

UniProt: P07766

Pathways: TCR Signaling, Ubiquitin Proteasome Pathway