CD3 anticorps (Activation Site)

N° du produit ABIN400888

Cet anticorps Souris Monoclonal détecte spécifiquement CD3 dans FACS, IP, IHC (fro), IF et WB. Il présente une réactivité envers Humain et Souris.

Aperçu rapide pour CD3 anticorps (Activation Site) (ABIN400888)

Antigène: CD3

Reactivité: Humain, Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD3 est non-conjugé

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Western Blotting (WB)

Clone: APA1-1

Détail du produit anti-CD3 anticorps

Épitope: Activation Site

Specificité: The antibody recognizes an activation-dependent intracellular epitope of CD3 epsilon. Exposure of the epitope precedes CD3 phosphorylation and recruitment and activation of ZAP70, which initiates the signaling cascade produced by T-cell activation. APA1/1 provides the earliest known marker for TCR-mediated T cell activation.

Réactivité croisée (Details): Species reactivity (tested):Human, Mouse

Attributs du produit: Synonyms: T3/Leu-4, T-cell surface glycoprotein CD3, T-cell surface antigen T3/Leu-4

Purification: Protein A affinity chromatography

Pureté: > 95 % by SDS-PAGE

Immunogène: Purified human CD3 proteins isolated from thymus

Isotype: IgG1

Information d'application

Indications d'application: Flow Cytometry. Sample preparation: At the end of stimulation of T cells, perform staining of surface markers (if required) in PBS+ 0.1 % BSA for 20 min. on ice. Wash with PBS and fix with 2 % formaldehyde, 30 min on ice. Wash with PBS and incubate in PBS + 0,1 % saponine, 5 min. RT. Incubate the cells in PBS + 1 % BSA + 0.03 % saponine, 15 min. on ice. Incubate with fluorescence-labeled APA1/1 antibody (1-5 μg/mL) in PBS + 1 % BSA + 0.03 %saponine in dark, 20 min. RT. Wash with PBS + 1 % BSA + 0.03 % saponine, resuspend in PBS. Positive control: Human T cells stimulated with anti-CD3 (MEM-57) antibody (1 μg/mL). Immunoprecipitation.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4, 15 mM sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C/-20 °C

Stockage commentaire: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C. Avoid repeated freezing andthawing.
Shelf life: one year from despatch.

Date de péremption: 12 months

Détails sur CD3

Antigène: CD3

Autre désignation: CD3

Sujet: CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation.Synonyms: T-cell surface antigen T3/Leu-4, T-cell surface glycoprotein CD3, T3/Leu-4

ID gène: 916

UniProt: P07766

Pathways: TCR Signaling, Ubiquitin Proteasome Pathway