UBE2N anticorps (AA 40-51)

N° du produit ABIN401175

Cet anticorps anti-UBE2N Polyclonal Chèvre (ABIN401175) détecte spécifiquement UBE2N dans WB et EIA. L’anticorps est réactif avec des échantillons de Humain, Rat, Souris, Poisson zèbre (Danio rerio), Chien, Boeuf (Vache), Poulet, Xenopus laevis, Chimpanzé et Macaque.

Aperçu rapide pour UBE2N anticorps (AA 40-51) (ABIN401175)

Antigène: UBE2N (Ubiquitin-Conjugating Enzyme E2N (UBE2N))

Reactivité: Humain, Rat, Souris, Poisson zèbre (Danio rerio), Chien, Boeuf (Vache), Poulet, Xenopus laevis, Chimpanzé, Macaque

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp UBE2N est non-conjugé

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Détail du produit anti-UBE2N anticorps

Épitope: AA 40-51

Specificité: This antibody is directed against UBC13 protein.

Réactivité croisée (Details): Species reactivity (expected):Canine, Bovine, Xenopus laevis, Xenopus tropicalis, Zebrafish, Rat, Mouse, Chimpanzee, Macaque, Chicken.
Species reactivity (tested):Human.

Purification: Affinity chromatography

Immunogène: Synthetic peptide corresponding to amino acids 40-51 of human UBC13 protein

Isotype: IgG

Information d'application

Indications d'application: ELISA: 1/5,000 - 1/25,000. Western Blot: 1/500 - 1/2,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.25 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: -20 °C

Détails sur UBE2N

Antigène: UBE2N (Ubiquitin-Conjugating Enzyme E2N (UBE2N))

Sujet: UBC13 is a member of the E2 ubiquitin-conjugating enzyme family also known as Ubiquitin-conjugating enzyme E2 N, Ubiquitin-protein ligase N, Ubiquitin carrier protein N and Bendless-like ubiquitin-conjugating enzyme. The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. UBC13 forms a heterodimer with UBE2V2 which then catalyzes the synthesis of non-canonical poly-ubiquitin chains that are linked through Lys-63. This type of polyubiquitination does not lead to protein degradation by the proteasome. UBC13 also mediates transcriptional activation of target genes and plays a role in the control of progress through the cell cycle and differentiation, as well as error-free DNA repair. UBC13 also contributes to the survival of cells after DNA damage.Synonyms: Bendless-like ubiquitin-conjugating enzyme, UBC13, Ubiquitin carrier protein N, Ubiquitin-conjugating enzyme E2 N, Ubiquitin-protein ligase N

ID gène: 7334

NCBI Accession: NP_003339

UniProt: P61088

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades, Positive Regulation of Response to DNA Damage Stimulus, Ubiquitin Proteasome Pathway