PCNA anticorps (Internal Region)

N° du produit ABIN401372

L’anticorps anti-PCNA Polyclonal Lapin est utilisé pour la détection de PCNA dans des échantillons de Humain, Souris, Rat, Poulet, Singe, Chien, Boeuf (Vache), Xenopus laevis et Poisson. Il a été validé pour WB, IHC (p) et EIA.

Aperçu rapide pour PCNA anticorps (Internal Region) (ABIN401372)

Antigène: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Reactivité: Humain, Souris, Rat, Poulet, Singe, Chien, Boeuf (Vache), Xenopus laevis, Poisson

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PCNA est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Détail du produit anti-PCNA anticorps

Épitope: Internal Region

Specificité: This antibody is directed against PCNA protein.

Réactivité croisée (Details): Species reactivity (expected):Monkey, Canine, Mouse, Rat, Bovine, Chicken, Fish, Xenopus.
Species reactivity (tested):Human.

Purification: Immunoaffinity chromatography

Immunogène: Synthetic peptide corresponding to an internal region of human PCNA protein

Information d'application

Indications d'application: ELISA: 1/2,000 - 1/8,000. Western Blot: 1/500 - 1/2,000. Immunohistochemistry on Paraffin sections: 2.5 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.62 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for up to one month or (in aliquots)at-20 °C for longer.

Détails sur PCNA

Antigène: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Autre désignation: PCNA

Sujet: The proliferating cell nuclear antigen (PCNA) is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the processibility of DNA polymerase during elongation of the leading strand. PCNA is expressed in the nucleus of all proliferating cells and is pivotal for DNA synthesis and cell cycle progression. In response to DNA damage, PCNA is mono-ubiquitinated and is involved in mismatch-provoked excision. PCNA is a useful marker for DNA synthesis and is highly conserved among most species.Synonyms: Cyclin, Proliferating Cell Nuclear Antigen

ID gène: 9606

UniProt: P12004

Pathways: Telomere Maintenance, Réparation de l'ADN, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Autophagy