RRM2B anticorps (N-Term)

N° du produit ABIN401481

Cet anticorps anti-RRM2B Polyclonal Lapin (ABIN401481) détecte spécifiquement RRM2B dans WB et EIA. L’anticorps est réactif avec des échantillons de Humain.

Aperçu rapide pour RRM2B anticorps (N-Term) (ABIN401481)

Antigène: RRM2B (Ribonucleotide Reductase M2 B (TP53 Inducible) (RRM2B))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp RRM2B est non-conjugé

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Détail du produit anti-RRM2B anticorps

Épitope: N-Term

Specificité: This antibody is directed against RRM2B.

Réactivité croisée (Details): Species reactivity (expected):Chimpanzee, Orangutan, Macaque, Porcine, Drosophila.
Species reactivity (tested):Human.

Purification: Affinity Purified

Immunogène: Synthetic peptide corresponding to a region near the N-terminus of human RRM2B1 protein

Isotype: IgG

Information d'application

Indications d'application: ELISA: 1/3,000. Western Blot: 1,0 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.82 mg/mL (by UV absorbance at 280nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Détails sur RRM2B

Antigène: RRM2B (Ribonucleotide Reductase M2 B (TP53 Inducible) (RRM2B))

Autre désignation: RRM2B / P53R2

Sujet: RRM2B/p53-R2, or p53-inducible ribonucleotide reductase small subunit 2-like protein, is a member of a broad superfamily of ferritin-like di-ironcarboxylate proteins. The RRM2B protein is an enzyme that catalyzes the conversion of ribonucleotides to deoxyribonucleotides that are essential for DNA synthesis, and is found in all eukaryotes. RRM2B plays a pivotal role in cell survival by repairing damaged DNA in a p53/TP53-dependent manner. It supplies deoxyribonucleotides for DNA repair in cells arrested at G1 or G2. It contains an iron-tyrosyl free radical center required for catalysis, and forms an active ribonucleotide reductase (RNR) complex with RRM1 which is expressed both in resting and proliferating cells in response to DNA damage. It is a heterotetramer with a large (RRM1) subunit, and interacts with p53/TP53. The interaction with RRM1 occurs in response to DNA damage and results in its translocation from cytoplasm to nucleus. It is widely expressed at a high level in skeletal muscle and at a weak level in thymus, and expressed in epithelial dysplasias and squamous cell carcinoma. Defects in RRM2B are the cause of encephalomyopathic mitochondrial depletion syndrome with renal tubulopathy (EMDSRT). Mitochondrial DNA depletion syndrome (MDS) is a clinically heterogeneous group of disorders characterized by a reduction in mitochondrial DNA (mtDNA) copy number. The encephalomyopathic form with renal tubulopathy is presented with various combinations of hypotonia, tubulopathy, seizures, respiratory distress, diarrhea, and lactic acidosis.Synonyms: Ribonucleoside-diphosphate reductase subunit M2 B, TP53-inducible ribonucleotide reductase M2 B, p53-inducible ribonucleotide reductase small subunit 2-like protein

ID gène: 50484

NCBI Accession: NP_001165948

UniProt: Q7LG56

Pathways: Signalisation p53, Negative Regulation of intrinsic apoptotic Signaling