CCL2 anticorps

N° du produit ABIN453916

Cet anticorps Souris Monoclonal détecte spécifiquement CCL2 dans WB, EIA et Func. Il présente une réactivité avec des échantillons de Humain.

Aperçu rapide pour CCL2 anticorps (ABIN453916)

Antigène: CCL2 (Chemokine (C-C Motif) Ligand 2 (CCL2))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CCL2 est non-conjugé

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Functional Studies (Func)

Clone: S14

Détail du produit anti-CCL2 anticorps

Specificité: This MCP1 antibody reacts with natural and recombinant Human MCP-1. Does not react with Human Interleukin-8 (IL-8) and other Human cytokines tested such as Interleukin-1 beta (IL1 beta), Serum Amyloid A (SAA) and Epidermal Growth Factor (EGF).

Réactivité croisée (Details): Species reactivity (tested):Human.

Purification: Affinity Chromatography on Protein G.

Immunogène: Purified recombinant Human MCP1.

Isotype: IgG1

Information d'application

Indications d'application: ELISA: React with Human MCP-1. Can be used as a Capture antibody in Sandwich ELISAapplications for human MCP-1 detection in combination with a monoclonaltracer/detection antibody. Suggested Capture Coating Dose: 0.3 μg/well. Substrate: TMB. If the above suggested conditions are followed approximately 1.5 pg/mL ofMCP-1 in serum/plasma or 4 pg/mL of MCP-1 in medium can be detected with an assayrange of 0-1600 pg/mL. Neutralizing: Tests on the ability to inhibit monocyte chemotaxis toward 1 nM recombinanthuman MCP-1 in blindwell chambers showed that the antibodies were particularly effectiveat blocking MCP-1 activity at a concentration of 0.5 μg/mL. It was also found that S-14 couldinhibit the function of native MCP-1 at concentrations similar to inhibitory doses forrecombinant MCP-1. Western Blot: MCP1 antibody at 0.1-1.0 μg/mL will allow visualization of 0.1 μg/lane ofhuman MCP1.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore with double distillated water to adjust the final concentration to 1.00 mg/mL

Buffer: 0.01 M PBS, pH 7.2 without preservatives

Agent conservateur: Without preservative

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: -20 °C

Détails sur CCL2

Antigène: CCL2 (Chemokine (C-C Motif) Ligand 2 (CCL2))

Autre désignation: MCP1 / CCL2

Sujet: This chemotactic factor attracts monocytes and basophils but not neutrophils or eosinophils and augments monocyte anti-tumor activity. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. It may be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis.Synonyms: C-C motif chemokine 2, HC-11, HC11, MCAF, MCP-1, Monocyte chemoattractant protein 1, Monocyte chemotactic and activating factor, Monocyte chemotactic protein 1, Monocyte secretory protein JE, SCYA2, Small-inducible cytokine A2

ID gène: 6347

NCBI Accession: NP_002973

UniProt: P13500

Pathways: Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, ER-Nucleus Signaling, Unfolded Protein Response, Phosphorylation & l'infection par le SRAS-CoV-2