CRYAA anticorps

N° du produit ABIN453938

L’anticorps anti-CRYAA Monoclonal Souris est utilisé pour la détection de CRYAA dans des échantillons de Humain, Souris, Rat et Boeuf (Vache). Il a été validé pour WB et EIA.

Aperçu rapide pour CRYAA anticorps (ABIN453938)

Antigène: CRYAA (Crystallin, alpha A (CRYAA))

Reactivité: Humain, Souris, Rat, Boeuf (Vache)

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CRYAA est non-conjugé

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Clone: 1H3-B8

Détail du produit anti-CRYAA anticorps

Specificité: This antibody recognizes Alpha A Crystallin at ~20 kDa. Detects alpha Crystallin complex (containing Alpha A and Alpha B subunits). Does not cross react with Alpha B Crystallin, Beta-L Crystallin, Beta-H Crystallin, Gamma Crystallin, Hsp25, Hsp27 or Hsp47 proteins.

Réactivité croisée (Details): Species reactivity (tested):Human, Mouse, Cow, Rat.

Attributs du produit: Synonyms: CRYAA, CRYA1, HSPB4, Heat shock protein beta-4

Purification: Protein G affinity chroamtography

Immunogène: Native alpha crystallin

Isotype: IgG1

Information d'application

Indications d'application: Western blot: 1/2000. ELISA.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: PBS pH 7.2, 0.09 % Sodium Azide, 50 % Glycerol

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C/-20 °C

Stockage commentaire: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C. Avoid repeated freezing andthawing.
Shelf life: one year from despatch.

Date de péremption: 12 months

Détails sur CRYAA

Antigène: CRYAA (Crystallin, alpha A (CRYAA))

Autre désignation: alpha-Crystallin A Chain

Sujet: The alpha-crystallins are major water-soluble lens structural proteins of the vertebrate eye that are related to the small heat shock protein family. The alphacrystallins possess structural and functional similarities with Hsp25 and Hsp27 (1). Mammalian lens cystallins are divided into alpha, beta and gamma families. Alpha and beta families are further divided into acidic and basic groups (Alpha-A and Alpha-B respectively). In the lens, alpha-crystallin primarily functions to maintain proper refractive index, however it can also function as a molecular chaperone that binds to the denatured proteins, keeping them in solution and thereby maintaining the translucency of the lens. When cellular stress occurs, alpha-crystallin enters its' phosphorylated state and may serve a structural control function and play a role in protein maintenance (2). In addition to their interaction with proteins, alpha-crystallins also interact with native molecules such as membrane proteins, Golgi matrix protein, structural proteins, nuclear proteins and DNA (3, 4, 5, 6, and 7). Two other functions are an autokinase activity and participation in the intracellular architecture, and it has also been proven that both alpha-A and B prevent apoptosis by inhibiting caspases (8).Synonyms: CRYA1, CRYAA, HSPB4, Heat shock protein beta-4

ID gène: 1409

UniProt: P02489

Pathways: M Phase